|
| Status |
Public on Aug 06, 2015 |
| Title |
025C-A |
| Sample type |
RNA |
| |
|
| Source name |
yeast cells
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
overexpression: GST-med3
|
| Growth protocol |
Yeast cells were grown SC-ura plus galactose medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from yeast cells according to Xue et al. 2004 (Yeast 21(1):25-39).
|
| Label |
Biotin
|
| Label protocol |
Labeling was performed according to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
|
| |
|
| Hybridization protocol |
According to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
|
| Scan protocol |
According to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
|
| Data processing |
Raw data (CEL files) was imported into ArrayStar 4, per-gene normalized to median and filtered on flags.
|
| |
|
| Submission date |
Feb 05, 2015 |
| Last update date |
Aug 06, 2015 |
| Contact name |
Claes M Gustafsson |
| E-mail(s) |
claes.gustafsson@medkem.gu.se
|
| Organization name |
Gothenburg University
|
| Department |
Department of Medical biochemistry and cell biology
|
| Street address |
Medcinaregatan 9A
|
| City |
Gothenburg |
| ZIP/Postal code |
405 30 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE65666 |
Expression profile in Overexpression of GST,GST-Med3 |
|
