|
| Status |
Public on May 29, 2015 |
| Title |
MI P20 #3 |
| Sample type |
genomic |
| |
|
| Source name |
Human intestinal stem cells_pedigree 2_Passage 20
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human intestinal stem cell
pedigree: Pedigree 2
passage number: Passage 20
|
| Treatment protocol |
Two independent pedigrees were isolated and collected at passage 5, 10, 15 and 20.
|
| Growth protocol |
Intestinal stem cells from human fetus (middle ileum) were cultured on 3T3 feeder layer.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA from cells were extracted using DNeasy blood and tissue kit (Qiagen)
|
| Label |
cy3, cy5
|
| Label protocol |
Followed Illumina standard protocol
|
| |
|
| Hybridization protocol |
Followed Illumina standard protocol
|
| Scan protocol |
Followed Illumina standard protocol
|
| Description |
Genomic DNA extracted from intestinal stem cells was genotyped using HumanOmniExpress BeadChip Kit (Illumina).
HumanOmniExpress BeadChip Kit
|
| Data processing |
GenomeStudio Software (Illumina)
|
| |
|
| Submission date |
Mar 10, 2015 |
| Last update date |
May 30, 2015 |
| Contact name |
Frank McKeon |
| E-mail(s) |
mckeon.xian@gmail.com
|
| Phone |
8608372486
|
| Organization name |
University of Houston
|
| Department |
Biology and Biochemistry
|
| Street address |
3455 Cullen Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77004 |
| Country |
USA |
| |
|
| Platform ID |
GPL19699 |
| Series (2) |
| GSE66747 |
Copy number variation of human intestinal stem cells with different passage in culture (two pedigrees, passage 5, 10, 15 and 20) |
| GSE66749 |
Cloning and Variation of Ground State Intestinal Stem Cells Super [expression & SNP studies] |
|
