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Sample GSM1652587 Query DataSets for GSM1652587
Status Public on Jul 28, 2022
Title control cerebellar tissue, biological rep7
Sample type RNA
 
Source name control cerebellar tissue
Organism Mus musculus
Characteristics strain: C57BL6
tissue: cerebellar tissue
age: Embryonic day 14.5
genotype: control
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed using RNeasy® Mini Kit (Qiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix small sample amplification protocol from app. 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description Gene expression data from control cerebellar tissue
Data processing Affymetrix raw data was normalized using the PLIER algorithms (Affymetrix Technical Note. Guide to Probe Logarithmic Intensity Error Estimation. http://affymetrix.com/support/technical/technotesmain.affx). The data from the three comparison groups were from three batches of Affymetrix experiments, and were normalized separately.
 
Submission date Apr 07, 2015
Last update date Jul 28, 2022
Contact name L. H.
Organization name Uppsala University
Street address Dag Hammarskjölds Väg 20
City Uppsala
ZIP/Postal code 751 85
Country Sweden
 
Platform ID GPL6246
Series (1)
GSE77049 Cerebellar gene expression changes in Pdgfc and Pdgfra mutant

Data table header descriptions
ID_REF
VALUE PLIER processed signal intensity

Data table
ID_REF VALUE
10338001 3458.15
10338002 158.7
10338003 1158.36
10338004 798.34
10338005 1.58
10338006 2.04
10338007 4.47
10338008 8.9
10338009 633.09
10338010 1.32
10338011 68.08
10338012 1.88
10338013 0
10338014 0.27
10338015 0.41
10338016 359.81
10338017 6972.94
10338018 199.36
10338019 42.13
10338020 397.06

Total number of rows: 35557

Table truncated, full table size 522 Kbytes.




Supplementary file Size Download File type/resource
GSM1652587_JA2.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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