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Sample GSM1655722 Query DataSets for GSM1655722
Status Public on Aug 01, 2016
Title conventional cultiver soybean diet, bio rep3
Sample type RNA
 
Source name SD rat liver, male
Organism Rattus norvegicus
Characteristics tissue: liver
strain: Male Sprague-Dawley rats
age: 4 weeks of age
diet: rats were adapted to the condition for 5 days and fed conventional cultiver soybean diet for 21 days
Treatment protocol Animals were randomly divided into 4 groups and fed an experimental diet for 21 days. The experimental diets were as follows: a diet containing 200 g/kg casein (Cas) ;a diet containing two proteins derived equally from casein and Fukuyutaka (conventional cultiver soybean) (F10); a diet containing two proteins derived from casein and Yukipirika (hihg-isoflavone soybean) in the proportion of three parts to one (Y05); a diet containing two proteins derived equally from casein and Yukipirika (Y10).
Growth protocol Male Sprague-Dawley rats were obtained from Charles River Japan (Kanagawa, Japan) at 4 week of age. Animals were housed individually in a room with a controlled temperature (20-22ºC), humidity (55-65%), and 12-h-light/-dark cycle. During their adaptation to the conditions, rats were fed a commercial non-purified diet (Type NMF; Oriental Yeast Co., Tokyo, Japan) for 5 days. Rats were allowed free access to diet and water.
Extracted molecule total RNA
Extraction protocol Acid guanidinium thiocyanate-phenol-chloroform extraction
Label biotin
Label protocol GeneChip® 3' IVT Express Kit
 
Hybridization protocol GeneChip® Hybridization Oven 640 (Affymetrix)
Scan protocol GeneChip® Scanner 3000 7G (Affymetrix)
Description normalized by SuperNORM data service
Data processing The perfect match data were normalized by using the SuperNORM data service (Skylight Biotech Inc., Akita, Japan) according to a three-parameter lognormal distribution model.
 
Submission date Apr 12, 2015
Last update date Aug 01, 2016
Contact name Tomokazu Konishi
E-mail(s) konishi@akita-pu.ac.jp
Phone +81-18-872-1603
Organization name Akita Prefectural University
Department Bioresource Sciences
Lab Molecular Genetics
Street address Shimoshinjyo Nishi
City Akita
State/province Akita
ZIP/Postal code 010-0195
Country Japan
 
Platform ID GPL1355
Series (1)
GSE67782 The effect of dietary soybeans and their isoflavone content on the expression profiles of mRNAs in liver of rats

Data table header descriptions
ID_REF
VALUE parametric: z-score of logarithms

Data table
ID_REF VALUE
1367452_at 1.56117373
1367453_at 1.517569967
1367454_at 1.48701785
1367455_at 1.782272411
1367456_at 2.007677631
1367457_at 1.148200176
1367458_at 1.053948767
1367459_at 2.18084427
1367460_at 1.677941494
1367461_at 1.113546677
1367462_at 1.594405683
1367463_at 1.854630755
1367464_at 1.326480339
1367465_at 1.796363473
1367466_at 1.44210377
1367467_at 1.910603618
1367468_at 1.519320698
1367469_at 1.904263949
1367470_at 1.405844999
1367471_at 1.316451262

Total number of rows: 31099

Table truncated, full table size 663 Kbytes.




Supplementary file Size Download File type/resource
GSM1655722_F10-4_Liv_131213.CEL.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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