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Sample GSM1655725 Query DataSets for GSM1655725
Status Public on Aug 01, 2016
Title casein+high-isoflavone soybean diet, bio rep1
Sample type RNA
 
Source name SD rat liver, male
Organism Rattus norvegicus
Characteristics tissue: liver
strain: Male Sprague-Dawley rats
age: 4 weeks of age
diet: rats were adapted to the condition for 5 days and fed casein+high-isoflavone soybean diet for 21 days
Treatment protocol Animals were randomly divided into 4 groups and fed an experimental diet for 21 days. The experimental diets were as follows: a diet containing 200 g/kg casein (Cas) ;a diet containing two proteins derived equally from casein and Fukuyutaka (conventional cultiver soybean) (F10); a diet containing two proteins derived from casein and Yukipirika (hihg-isoflavone soybean) in the proportion of three parts to one (Y05); a diet containing two proteins derived equally from casein and Yukipirika (Y10).
Growth protocol Male Sprague-Dawley rats were obtained from Charles River Japan (Kanagawa, Japan) at 4 week of age. Animals were housed individually in a room with a controlled temperature (20-22ºC), humidity (55-65%), and 12-h-light/-dark cycle. During their adaptation to the conditions, rats were fed a commercial non-purified diet (Type NMF; Oriental Yeast Co., Tokyo, Japan) for 5 days. Rats were allowed free access to diet and water.
Extracted molecule total RNA
Extraction protocol Acid guanidinium thiocyanate-phenol-chloroform extraction
Label biotin
Label protocol GeneChip® 3' IVT Express Kit
 
Hybridization protocol GeneChip® Hybridization Oven 640 (Affymetrix)
Scan protocol GeneChip® Scanner 3000 7G (Affymetrix)
Description normalized by SuperNORM data service
Data processing The perfect match data were normalized by using the SuperNORM data service (Skylight Biotech Inc., Akita, Japan) according to a three-parameter lognormal distribution model.
 
Submission date Apr 12, 2015
Last update date Aug 01, 2016
Contact name Tomokazu Konishi
E-mail(s) konishi@akita-pu.ac.jp
Phone +81-18-872-1603
Organization name Akita Prefectural University
Department Bioresource Sciences
Lab Molecular Genetics
Street address Shimoshinjyo Nishi
City Akita
State/province Akita
ZIP/Postal code 010-0195
Country Japan
 
Platform ID GPL1355
Series (1)
GSE67782 The effect of dietary soybeans and their isoflavone content on the expression profiles of mRNAs in liver of rats

Data table header descriptions
ID_REF
VALUE parametric: z-score of logarithms

Data table
ID_REF VALUE
1367452_at 1.613580907
1367453_at 1.574121954
1367454_at 1.531780514
1367455_at 1.862379954
1367456_at 1.997600644
1367457_at 1.126787436
1367458_at 1.062429849
1367459_at 2.196545538
1367460_at 1.704590644
1367461_at 1.148000031
1367462_at 1.586098723
1367463_at 1.773818212
1367464_at 1.365567418
1367465_at 1.745853967
1367466_at 1.44458394
1367467_at 1.955486806
1367468_at 1.447241618
1367469_at 1.943133301
1367470_at 1.428375553
1367471_at 1.223971451

Total number of rows: 31099

Table truncated, full table size 663 Kbytes.




Supplementary file Size Download File type/resource
GSM1655725_Y05-1_Liv_131212.CEL.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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