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Status |
Public on Aug 01, 2016 |
Title |
casein+high-isoflavone soybean diet, bio rep1 |
Sample type |
RNA |
|
|
Source name |
SD rat liver, male
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: liver strain: Male Sprague-Dawley rats age: 4 weeks of age diet: rats were adapted to the condition for 5 days and fed casein+high-isoflavone soybean diet for 21 days
|
Treatment protocol |
Animals were randomly divided into 4 groups and fed an experimental diet for 21 days. The experimental diets were as follows: a diet containing 200 g/kg casein (Cas) ;a diet containing two proteins derived equally from casein and Fukuyutaka (conventional cultiver soybean) (F10); a diet containing two proteins derived from casein and Yukipirika (hihg-isoflavone soybean) in the proportion of three parts to one (Y05); a diet containing two proteins derived equally from casein and Yukipirika (Y10).
|
Growth protocol |
Male Sprague-Dawley rats were obtained from Charles River Japan (Kanagawa, Japan) at 4 week of age. Animals were housed individually in a room with a controlled temperature (20-22ºC), humidity (55-65%), and 12-h-light/-dark cycle. During their adaptation to the conditions, rats were fed a commercial non-purified diet (Type NMF; Oriental Yeast Co., Tokyo, Japan) for 5 days. Rats were allowed free access to diet and water.
|
Extracted molecule |
total RNA |
Extraction protocol |
Acid guanidinium thiocyanate-phenol-chloroform extraction
|
Label |
biotin
|
Label protocol |
GeneChip® 3' IVT Express Kit
|
|
|
Hybridization protocol |
GeneChip® Hybridization Oven 640 (Affymetrix)
|
Scan protocol |
GeneChip® Scanner 3000 7G (Affymetrix)
|
Description |
normalized by SuperNORM data service
|
Data processing |
The perfect match data were normalized by using the SuperNORM data service (Skylight Biotech Inc., Akita, Japan) according to a three-parameter lognormal distribution model.
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|
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Submission date |
Apr 12, 2015 |
Last update date |
Aug 01, 2016 |
Contact name |
Tomokazu Konishi |
E-mail(s) |
konishi@akita-pu.ac.jp
|
Phone |
+81-18-872-1603
|
Organization name |
Akita Prefectural University
|
Department |
Bioresource Sciences
|
Lab |
Molecular Genetics
|
Street address |
Shimoshinjyo Nishi
|
City |
Akita |
State/province |
Akita |
ZIP/Postal code |
010-0195 |
Country |
Japan |
|
|
Platform ID |
GPL1355 |
Series (1) |
GSE67782 |
The effect of dietary soybeans and their isoflavone content on the expression profiles of mRNAs in liver of rats |
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