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Sample GSM1655726 Query DataSets for GSM1655726
Status Public on Aug 01, 2016
Title casein+high-isoflavone soybean diet, bio rep2
Sample type RNA
Source name SD rat liver, male
Organism Rattus norvegicus
Characteristics tissue: liver
strain: Male Sprague-Dawley rats
age: 4 weeks of age
diet: rats were adapted to the condition for 5 days and fed casein+high-isoflavone soybean diet for 21 days
Treatment protocol Animals were randomly divided into 4 groups and fed an experimental diet for 21 days. The experimental diets were as follows: a diet containing 200 g/kg casein (Cas) ;a diet containing two proteins derived equally from casein and Fukuyutaka (conventional cultiver soybean) (F10); a diet containing two proteins derived from casein and Yukipirika (hihg-isoflavone soybean) in the proportion of three parts to one (Y05); a diet containing two proteins derived equally from casein and Yukipirika (Y10).
Growth protocol Male Sprague-Dawley rats were obtained from Charles River Japan (Kanagawa, Japan) at 4 week of age. Animals were housed individually in a room with a controlled temperature (20-22ºC), humidity (55-65%), and 12-h-light/-dark cycle. During their adaptation to the conditions, rats were fed a commercial non-purified diet (Type NMF; Oriental Yeast Co., Tokyo, Japan) for 5 days. Rats were allowed free access to diet and water.
Extracted molecule total RNA
Extraction protocol Acid guanidinium thiocyanate-phenol-chloroform extraction
Label biotin
Label protocol GeneChip® 3' IVT Express Kit
Hybridization protocol GeneChip® Hybridization Oven 640 (Affymetrix)
Scan protocol GeneChip® Scanner 3000 7G (Affymetrix)
Description normalized by SuperNORM data service
Data processing The perfect match data were normalized by using the SuperNORM data service (Skylight Biotech Inc., Akita, Japan) according to a three-parameter lognormal distribution model.
Submission date Apr 12, 2015
Last update date Aug 01, 2016
Contact name Tomokazu Konishi
Phone +81-18-872-1603
Organization name Akita Prefectural University
Department Bioresource Sciences
Lab Molecular Genetics
Street address Shimoshinjyo Nishi
City Akita
State/province Akita
ZIP/Postal code 010-0195
Country Japan
Platform ID GPL1355
Series (1)
GSE67782 The effect of dietary soybeans and their isoflavone content on the expression profiles of mRNAs in liver of rats

Data table header descriptions
VALUE parametric: z-score of logarithms

Data table
1367452_at 1.670598025
1367453_at 1.544919218
1367454_at 1.535911757
1367455_at 1.896368647
1367456_at 2.029662301
1367457_at 1.199601525
1367458_at 1.077166426
1367459_at 2.232114126
1367460_at 1.745948173
1367461_at 1.139002242
1367462_at 1.600050777
1367463_at 1.844133809
1367464_at 1.417404462
1367465_at 1.766778468
1367466_at 1.413702396
1367467_at 1.9414048
1367468_at 1.539533316
1367469_at 2.060003845
1367470_at 1.415197231
1367471_at 1.23565582

Total number of rows: 31099

Table truncated, full table size 663 Kbytes.

Supplementary file Size Download File type/resource
GSM1655726_Y05-2_Liv_131213.CEL.gz 2.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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