|
| Status |
Public on Apr 21, 2015 |
| Title |
5823_before |
| Sample type |
RNA |
| |
|
| Source name |
PBMC cells
|
| Organism |
Homo sapiens |
| Characteristics |
time: before treatment
treatment: baseline
cell type: PBMC
|
| Treatment protocol |
Human obese patients before and during treatment with rimonabant
|
| Growth protocol |
Ficoll separated peripheral blood mononuclear cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen Mini Plus kit
|
| Label |
biotin
|
| Label protocol |
GeneChip WT cDNA Synthesis and Amplification kit and GeneChip WT Terminal labeling kit
|
| |
|
| Hybridization protocol |
Targets were hybridized in Affymetrix GeneChip Hybridization oven 640 and washed and stained in Affymetrix Fluidics station 450
|
| Scan protocol |
Arrays were scanned in Affymetrix GeneChip scanner 3000 7g
|
| Description |
MN05_5823B (before treatment)
|
| Data processing |
The data were analyzed with Affymetrix Expression Console (vesion 1.4.1.46) using the default RMA analysis method
|
| |
|
| Submission date |
Apr 20, 2015 |
| Last update date |
Apr 21, 2015 |
| Contact name |
Birgitta Sander |
| E-mail(s) |
birgitta.sander@ki.se
|
| Organization name |
Karolinska Institutet
|
| Street address |
F46
|
| City |
Stockholm |
| ZIP/Postal code |
SE 141 86 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE68055 |
Gene expression in obese humans before and during treatment with rimonabant |
|
