|
| Status |
Public on May 16, 2015 |
| Title |
WT_MIR replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
WT_MIR
|
| Organism |
Mus musculus |
| Characteristics |
age: adult
tissue: hippocampus
condition: euploid
transduction: Lv-miR155-802T
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were prepared with Qiagen Rneasy minikit according to manufacturer’s protocol
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA from each sample were amplified by Oligo-dT-T7 reverse transcription and labeled by in vitro transcription with T7 RNA polymerase in the presence of Cy3-CTP using the LowInputQuick Amp Labeling kit (Agilent) and purified using RNAeasy columns (Qiagen, Hilden, Germany)
|
| |
|
| Hybridization protocol |
After fragmentation, 600 ng of labeled cRNA from each sample was hybridized in in situ hybridization oven (Agilent) for 17 h at 65ºC and washed during 1 min at rt in Gene Expression Wash Buffer 1 (Agilent) and 1 min at 37 ºC with Gene Expression Wahs buffer 2 (Agilent).
|
| Scan protocol |
Scanned on an Agilent G2539A scanner at 5um resolution and 100%PMT
The intensity data of each individual hybridization were extracted and the quality was assessed with the Feature Extraction software 10.7 (Agilent).
|
| Data processing |
Raw data was corrected for background noise using the normexp method. Quantile normalization was applied to assure comparability across samples.
|
| |
|
| Submission date |
Apr 20, 2015 |
| Last update date |
May 16, 2015 |
| Contact name |
Xavier Bofill-De Ros |
| E-mail(s) |
xbofill@mbg.au.dk
|
| Phone |
+13018465576
|
| Organization name |
National Cancer Institute
|
| Department |
RNA Biology Laboratory
|
| Lab |
RNA mediated Gene Regulation
|
| Street address |
1050 Boyles St., blg 560
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE68074 |
Lentiviral vector-based microRNA sponges identifies miR-155 and miR-802 target genes in the hippocampus of Ts65Dn mice |
|
