|
| Status |
Public on May 05, 2015 |
| Title |
hESC-KCL038 |
| Sample type |
genomic |
| |
|
| Source name |
Human embryonic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: embryonic stem cells
gender: Male
passage: passage 9
|
| Treatment protocol |
Self-renewing conditions.
|
| Growth protocol |
Various self-renewing media and matrices. Media included Essential 8, StemPro, or NutriStem, and matrices included CellStart or Laminin-521.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated from cell pellets using the MasterPure™ Complete DNA and RNA Purification Kit (Epicentre). Briefly, hES cell pellets were lysed with Tissue and Cell Lysis Solution, followed by Proteinase K and RNase A treatment. Proteins were precipitated with MPC Protein Precipitation Reagent and removed by centrifugation. Genomic DNA was precipitated with isopropanol, pelleted by centrifugation and then resuspended in TE buffer to a final concentration of 50 ng/μl. Purity was checked by spectrophotometry using the NanoDrop 1000 spectrophotometer (Thermo Fisher).
|
| Label |
C/G-biotin and A/T-DNP
|
| Label protocol |
200ng of genomic DNA (quantified by the PicoGreen assay) was labelled as per the Infinium HD Assay Ultra Protocol Guide
|
| |
|
| Hybridization protocol |
Labelled fragments were hybridized to Multi BeadChips as per the Infinium HD Assay Ultra Protocol Guide
|
| Scan protocol |
Hybridized beads were scanned with the iScan system and the BeadArray Reader
|
| Description |
Genomic DNA extracted from hES cells was genotyped using Illumina HumanCytoSNP-12 v2.1 BeadChip arrays
|
| Data processing |
Illumina GenomeStudio (v1.94)
|
| |
|
| Submission date |
May 04, 2015 |
| Last update date |
May 05, 2015 |
| Contact name |
Tilo Kunath |
| E-mail(s) |
tilo.kunath@ed.ac.uk
|
| Phone |
+44 (0) 131 651 9500
|
| Organization name |
University of Edinburgh
|
| Department |
Centre for Regenerative Medicine
|
| Street address |
5 LIttle France Drive
|
| City |
Edinburgh |
| ZIP/Postal code |
EH16 4UU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13829 |
| Series (1) |
| GSE68508 |
Molecular karyotype analysis of 25 clinical-grade human embryonic stem cell lines |
|
