|
Status |
Public on Jun 12, 2017 |
Title |
WT untreated replicate 1 |
Sample type |
RNA |
|
|
Source name |
Mouse embryonic fibroblasts
|
Organism |
Mus musculus |
Characteristics |
cell type: Mouse embryonic fibroblasts genotype: WT treatment: untreated strain: C57BL/6
|
Treatment protocol |
Mouse embryonic fibroblasts were treated at 90% confluency with recombinant tumor necrosis factor (10 ng/ml)
|
Growth protocol |
Mouse embryonic fibroblasts were cultured in complete DMEM media with 10% BCS and passaged by trypsinizing every third day.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using RNeasy Kit (QIAGEN) and on column DNase digestion was performed according to manufacturer protocol. Quality control was performed using Agilent Bioanalyzer.
|
Label |
Cy3
|
Label protocol |
Biotinylated cRNA were prepared with TargetAmpTM- Nano labelling Kit for illumina Expression Beadchip
|
|
|
Hybridization protocol |
illumina standard direct hybridization protocol
|
Scan protocol |
By using illumina Bead Array Reader version 3.7.9.27572
|
Description |
SAMPLE 1
|
Data processing |
The raw data was extracted from illumina's Genome studio software and the same used to check the quality of data. The data were normalized using quantile normalisation method.
|
|
|
Submission date |
May 06, 2015 |
Last update date |
Jun 19, 2017 |
Contact name |
Soumen Basak |
Organization name |
National Institute of Immunology
|
Department |
Infection and immunity
|
Lab |
Systems Immunology Laboratory
|
Street address |
Aruna Asaf Ali Marg
|
City |
New Delhi |
State/province |
Delhi |
ZIP/Postal code |
110067 |
Country |
India |
|
|
Platform ID |
GPL6885 |
Series (2) |
GSE68615 |
An autoregulatory RelB:p50 NF-κB pathway perpetuates pro-survival TNF response in multiple myeloma |
GSE99980 |
TNF-induced gene-expressions in Nfkb2-/- MEFs and its derivatives |
|