|
| Status |
Public on Jun 12, 2017 |
| Title |
WT 6h TNF replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Mouse embryonic fibroblasts
genotype: WT
treatment: TNF
strain: C57BL/6
|
| Treatment protocol |
Mouse embryonic fibroblasts were treated at 90% confluency with recombinant tumor necrosis factor (10 ng/ml)
|
| Growth protocol |
Mouse embryonic fibroblasts were cultured in complete DMEM media with 10% BCS and passaged by trypsinizing every third day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy Kit (QIAGEN) and on column DNase digestion was performed according to manufacturer protocol. Quality control was performed using Agilent Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with TargetAmpTM- Nano labelling Kit for illumina Expression Beadchip
|
| |
|
| Hybridization protocol |
illumina standard direct hybridization protocol
|
| Scan protocol |
By using illumina Bead Array Reader version 3.7.9.27572
|
| Description |
SAMPLE 4
|
| Data processing |
The raw data was extracted from illumina's Genome studio software and the same used to check the quality of data. The data were normalized using quantile normalisation method.
|
| |
|
| Submission date |
May 06, 2015 |
| Last update date |
Jun 19, 2017 |
| Contact name |
Soumen Basak |
| Organization name |
National Institute of Immunology
|
| Department |
Infection and immunity
|
| Lab |
Systems Immunology Laboratory
|
| Street address |
Aruna Asaf Ali Marg
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110067 |
| Country |
India |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE68615 |
An autoregulatory RelB:p50 NF-κB pathway perpetuates pro-survival TNF response in multiple myeloma |
| GSE99980 |
TNF-induced gene-expressions in Nfkb2-/- MEFs and its derivatives |
|
