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Sample GSM1700918 Query DataSets for GSM1700918
Status Public on Aug 10, 2016
Title can mutant small RNA-Seq
Sample type SRA
Source name testis
Organism Drosophila melanogaster
Characteristics cell type: primary spermatocytes
Extracted molecule total RNA
Extraction protocol RNA extraction from hand dissected testes
True-seq small RNA library construction
Library strategy ncRNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina HiSeq 2000
Description The apical tips were removed from testis to remove early mitotically dividing germline cells
Data processing bad quality read removal -Fastq toolkit
Alignment to genome - Bowtie (v1)
Libarray Normalization - RLE method (DESeq2)
Obtaining normalized read data - DEseq2
Genome_build: Drosophila melanogaster rel 5.25
Supplementary_files_format_and_content: cluster and transposon mapping piRNAs
Submission date Jun 01, 2015
Last update date May 15, 2019
Contact name Toshie Kai
Organization name Temasek Life Sciences Laboratory
Street address 1 Research Link, National University of Singapore
City Singapore
ZIP/Postal code 117604
Country Singapore
Platform ID GPL13304
Series (1)
GSE69417 Developmental regulation of piRNAs during spermatogenesis in Drosophila melanogaster
BioSample SAMN03754040
SRA SRX1044739

Supplementary file Size Download File type/resource
GSM1700918_can_testis.xlsx 52.6 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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