|
| Status |
Public on Aug 10, 2016 |
| Title |
can mutant small RNA-Seq |
| Sample type |
SRA |
| |
|
| Source name |
testis
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: primary spermatocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction from hand dissected testes
True-seq small RNA library construction
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
The apical tips were removed from testis to remove early mitotically dividing germline cells
|
| Data processing |
bad quality read removal -Fastq toolkit
Alignment to genome - Bowtie (v1)
Libarray Normalization - RLE method (DESeq2)
Obtaining normalized read data - DEseq2
Genome_build: Drosophila melanogaster rel 5.25
Supplementary_files_format_and_content: cluster and transposon mapping piRNAs
|
| |
|
| Submission date |
Jun 01, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Toshie Kai |
| E-mail(s) |
toshie@tll.org.sg
|
| Organization name |
Temasek Life Sciences Laboratory
|
| Street address |
1 Research Link, National University of Singapore
|
| City |
Singapore |
| ZIP/Postal code |
117604 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL13304 |
| Series (1) |
| GSE69417 |
Developmental regulation of piRNAs during spermatogenesis in Drosophila melanogaster |
|
| Relations |
| BioSample |
SAMN03754040 |
| SRA |
SRX1044739 |
