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Sample GSM1700922 Query DataSets for GSM1700922
Status Public on Aug 10, 2016
Title ago3 (t2/t3) mutants small RNA-Seq
Sample type SRA
 
Source name testis
Organism Drosophila melanogaster
Characteristics cell type: gonioblasts to mature sperms
Extracted molecule total RNA
Extraction protocol RNA extraction from hand dissected testes
True-seq small RNA library construction
 
Library strategy ncRNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina HiSeq 2000
 
Description whole testis was used for RNA -extraction
Data processing bad quality read removal -Fastq toolkit
Alignment to genome - Bowtie (v1)
Libarray Normalization - RLE method (DESeq2)
Obtaining normalized read data - DEseq2
Genome_build: Drosophila melanogaster rel 5.25
Supplementary_files_format_and_content: cluster and transposon mapping piRNAs
 
Submission date Jun 01, 2015
Last update date May 15, 2019
Contact name Toshie Kai
E-mail(s) toshie@tll.org.sg
Organization name Temasek Life Sciences Laboratory
Street address 1 Research Link, National University of Singapore
City Singapore
ZIP/Postal code 117604
Country Singapore
 
Platform ID GPL13304
Series (1)
GSE69417 Developmental regulation of piRNAs during spermatogenesis in Drosophila melanogaster
Relations
BioSample SAMN03754044
SRA SRX1044743

Supplementary file Size Download File type/resource
GSM1700922_ago3_mutant_testis.xlsx 55.3 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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