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Status |
Public on Jun 03, 2015 |
Title |
control, biological rep 1 |
Sample type |
RNA |
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|
Source name |
BR5FVB1-Akt cells
|
Organism |
Mus musculus |
Characteristics |
cell line: BR5FVB1-Akt tissue type: ovarian cancer
|
Treatment protocol |
BR5FVB1-Akt cells were treated with 10 uM of decitabine for 72 hours.
|
Growth protocol |
BR5FVB1-Akt cells were cultured in DMEM with 10% FBS and 1% Penicillin/ Streptomycin at 37C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Qiagen RNeasy Mini Kit.
|
Label |
biotin
|
Label protocol |
The samples were processed following the Affymetrix GeneChip WT Plus Reagent Kit (cat # 703174), followed by the Affymetrix GeneChip WT Terminal Lebeling and Hybridization (cat# 702808) onto Affymetrix Mouse Gene 2.0ST arrays. the Affymetrix GeneChip WT Terminal Labeling.
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|
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Hybridization protocol |
Affymetrix GeneChip WT Terminal Lebeling and Hybridization (cat# 702808). the Affymetrix GeneChip WT Terminal Labeling.
|
Scan protocol |
The arrays were scanned on GeneChip Scanner 3000 7G.
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Description |
gene expression data from cultured BR5FVB1-Akt cells
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Data processing |
The data were analyzed using Affymetrix Transcroptome Analysis Console software.
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|
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Submission date |
Jun 02, 2015 |
Last update date |
Jun 03, 2015 |
Contact name |
Michael Solomon Goldberg |
E-mail(s) |
Michael_Goldberg1@DFCI.HARVARD.EDU
|
Organization name |
Dana-Farber Cancer Institute
|
Department |
Cancer Immunology and AIDS
|
Street address |
450 Brookline Avenue
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02215 |
Country |
USA |
|
|
Platform ID |
GPL16570 |
Series (1) |
GSE69454 |
Expression data from decitabine-treated and non-treated BR5FVB1-Akt cells |
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