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Sample GSM1701443 Query DataSets for GSM1701443
Status Public on Jun 03, 2015
Title control, biological rep 1
Sample type RNA
 
Source name BR5FVB1-Akt cells
Organism Mus musculus
Characteristics cell line: BR5FVB1-Akt
tissue type: ovarian cancer
Treatment protocol BR5FVB1-Akt cells were treated with 10 uM of decitabine for 72 hours.
Growth protocol BR5FVB1-Akt cells were cultured in DMEM with 10% FBS and 1% Penicillin/ Streptomycin at 37C.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Qiagen RNeasy Mini Kit.
Label biotin
Label protocol The samples were processed following the Affymetrix GeneChip WT Plus Reagent Kit (cat # 703174), followed by the Affymetrix GeneChip WT Terminal Lebeling and Hybridization (cat# 702808) onto Affymetrix Mouse Gene 2.0ST arrays. the Affymetrix GeneChip WT Terminal Labeling.
 
Hybridization protocol Affymetrix GeneChip WT Terminal Lebeling and Hybridization (cat# 702808). the Affymetrix GeneChip WT Terminal Labeling.
Scan protocol The arrays were scanned on GeneChip Scanner 3000 7G.
Description gene expression data from cultured BR5FVB1-Akt cells
Data processing The data were analyzed using Affymetrix Transcroptome Analysis Console software.
 
Submission date Jun 02, 2015
Last update date Jun 03, 2015
Contact name Michael Solomon Goldberg
E-mail(s) Michael_Goldberg1@DFCI.HARVARD.EDU
Organization name Dana-Farber Cancer Institute
Department Cancer Immunology and AIDS
Street address 450 Brookline Avenue
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL16570
Series (1)
GSE69454 Expression data from decitabine-treated and non-treated BR5FVB1-Akt cells

Data table header descriptions
ID_REF
VALUE log2 RMA

Data table
ID_REF VALUE
17200001 5.52505
17200003 5.58542
17200005 4.25124
17200007 4.30036
17200009 5.50687
17200011 4.86898
17200013 4.76825
17200015 5.55212
17200017 3.49546
17200019 3.38149
17200021 4.76476
17200023 5.81758
17200025 5.92747
17200027 5.97039
17200029 4.13246
17200031 2.67403
17200033 4.27571
17200035 2.38948
17200037 4.4665
17200039 2.5233

Total number of rows: 41345

Table truncated, full table size 681 Kbytes.




Supplementary file Size Download File type/resource
GSM1701443_37_control_1.1.rma-gene-full.chp.gz 254.5 Kb (ftp)(http) CHP
GSM1701443_37_control_1.CEL.gz 8.5 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data provided as supplementary file

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