|
| Status |
Public on Jun 03, 2015 |
| Title |
decitabine treatment, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
decitabine-treated BR5FVB1-Akt cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: BR5FVB1-Akt
tissue type: ovarian cancer
|
| Treatment protocol |
BR5FVB1-Akt cells were treated with 10 uM of decitabine for 72 hours.
|
| Growth protocol |
BR5FVB1-Akt cells were cultured in DMEM with 10% FBS and 1% Penicillin/ Streptomycin at 37C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy Mini Kit.
|
| Label |
biotin
|
| Label protocol |
The samples were processed following the Affymetrix GeneChip WT Plus Reagent Kit (cat # 703174), followed by the Affymetrix GeneChip WT Terminal Lebeling and Hybridization (cat# 702808) onto Affymetrix Mouse Gene 2.0ST arrays. the Affymetrix GeneChip WT Terminal Labeling.
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip WT Terminal Lebeling and Hybridization (cat# 702808). the Affymetrix GeneChip WT Terminal Labeling.
|
| Scan protocol |
The arrays were scanned on GeneChip Scanner 3000 7G.
|
| Description |
gene expression data from cultured BR5FVB1-Akt cells with dectabine treatment
|
| Data processing |
The data were analyzed using Affymetrix Transcroptome Analysis Console software.
|
| |
|
| Submission date |
Jun 02, 2015 |
| Last update date |
Jun 03, 2015 |
| Contact name |
Michael Solomon Goldberg |
| E-mail(s) |
Michael_Goldberg1@DFCI.HARVARD.EDU
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Cancer Immunology and AIDS
|
| Street address |
450 Brookline Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE69454 |
Expression data from decitabine-treated and non-treated BR5FVB1-Akt cells |
|
