|
| Status |
Public on Oct 29, 2015 |
| Title |
tonsil_total_epi_3_crypt |
| Sample type |
RNA |
| |
|
| Source name |
Palatine tonsils, epithelium, crypt
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: male
age: 24 yo
tissue: epithelium
cell type: total-epithelial-enriched
original id: 12289R2
location: crypt
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using AllPrep DNA/RNA Micro Kit (Qiagen) in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser 2100 using the Agilent RNA 6000 Nanokit or Picokit (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
70 ng of RNA was labeled with TotalTM-96 RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 10
|
| Data processing |
The data were normalised using quantile normalisation with Gene-E
|
| |
|
| Submission date |
Jun 11, 2015 |
| Last update date |
Oct 29, 2015 |
| Contact name |
Catherine Kang |
| E-mail(s) |
ckang@bccrc.ca
|
| Organization name |
British Columbia Cancer Research Centre
|
| Street address |
675 West 10th Avenue
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE69767 |
Gene expression profiles of normal human tonsillar cell subpopulations FACS sorted based on expression of cell surface markers |
|
