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Sample GSM1709

Query DataSets for GSM1709

Status

Public on Jun 27, 2002

Title

110 antigens in 100X FCS,mix#1

Sample type

protein

Channel 1

Source name

reference antigens

Organism

Extracted molecule

protein

Channel 2

Source name

test antigens

Organism

Extracted molecule

protein

Description

Category: Protein array
Subcategory: Antibody
Scanning Software: ScanAlyze
Software version: 2.33
Lot batch = 11cl52

Submission date

May 23, 2002

Last update date

Nov 21, 2005

Organization

Stanford Microarray Database (SMD)

E-mail(s)

array@genome.stanford.edu

Phone

650-498-6012

URL

http://genome-www5.stanford.edu/

Department

Stanford University, School of Medicine

Street address

300 Pasteur Drive

City

Stanford

State/province

CA

ZIP/Postal code

94305

Country

USA

Platform ID

Series (2)

GSE47	Antibody Arrays Figure 6 100X FCS
GSE50	Antibody Array Figures

Data table header descriptions
ID_REF
CH1B_MEAN	mean channel 1 background
CH1B_MEDIAN	median channel 1 background
CH1D_MEAN	difference between CH1I_MEAN and CH1B_MEAN
CH2I_MEAN	mean channel 2 signal
CH2D_MEAN	difference of CH2I_MEAN and CH2B_MEAN
CH2B_MEAN	mean of channel 2 background
CH2B_MEDIAN	median of channel 2 background
CH2BN_MEDIAN	normalized CH2B_MEDIAN
CH2DN_MEAN	normalized CH2D_MEAN
CH2IN_MEAN	normalized CH2I_MEAN
CORR	simple correlation coefficient of channel 1 and channel 2 pixels
FLAG	user defined flag (default=0)
VALUE	same as UNF_VALUE but with flagged values removed
PIX_RAT2_MEDIAN
PERGTBCH1I_1SD	standard deviation of fraction of pixels greater than CH1B_MEAN
PERGTBCH2I_1SD	standard deviation of fraction of pixels greater than CH2B_MEAN
RAT1_MEAN	ratio of CH1D_MEAN to CH2D_MEAN
RAT1N_MEAN	ratio of CH1DN_MEAN to CH2DN_MEAN
RAT2_MEAN	ratio of CH2D_MEAN to CH1D_MEAN
RAT2N_MEAN	ratio of CH2DN_MEAN and CH1DN_MEAN
REGR	slope of the simple regression line of channel 2 to channel 1 pixels
TOT_BPIX	total number of background pixels
TOT_SPIX	total number of signal (spot) pixels
TOP	vertical, short-axis spot ellipse minimum pixel coordinate
BOT	vertical, short-axis spot ellipse maximum pixel coordinate
LEFT	horizontal, long-axis spot ellipse minimum pixel coordinate
RIGHT	horizontal, long-axis spot ellipse maximum pixel coordinate
UNF_VALUE	aka LOG_RAT2N_MEAN, or log2 of ratio of CH2DN_MEAN and CH1DN_MEAN

Data table
ID_REF	CH1B_MEAN	CH1B_MEDIAN	CH1D_MEAN	CH2I_MEAN	CH2D_MEAN	CH2B_MEAN	CH2B_MEDIAN	CH2BN_MEDIAN	CH2DN_MEAN	CH2IN_MEAN	CORR	FLAG	VALUE	PIX_RAT2_MEDIAN	PERGTBCH1I_1SD	PERGTBCH2I_1SD	RAT1_MEAN	RAT1N_MEAN	RAT2_MEAN	RAT2N_MEAN	REGR	TOT_BPIX	TOT_SPIX	TOP	BOT	LEFT	RIGHT	UNF_VALUE
1	734	680	267	743		796	772	697	-26	671	.028	0		.301	38	0					.012	1544	137	261	274	163	176
2	717	682	494	759		776	774	699	-14	686	.045	0		.264	36	2					.015	1544	137	261	274	203	216
3	710	691	262	785		826	824	744	-35	709		0		.29	36	0						1544	137	261	274	243	256
4	784	714	183	924	95	843	829	749	86	835	.162	0	-1.093	.612	19	4	1.926	2.133	.519	.469	.088	1544	137	262	275	284	297	-1.093
5	931	692	272	952	109	900	843	761	98	860	.582	0	-1.466	.809	18	4	2.495	2.763	.401	.362	.104	1544	137	262	275	324	337	-1.466
6	781	724	395	1015	136	889	879	794	123	917	.108	0	-1.685	.92	22	7	2.904	3.215	.344	.311	.009	1544	137	263	276	364	377	-1.685
7	896	744	175	921		1088	922	833	-1	832	.229	0		.899	15	2					.374	1544	137	263	276	405	418
8	830	755	73	856		1008	1002	905	-132	773	.297	0		.95	5	0					.423	1544	137	264	277	445	458
9	762	752	98	868		989	982	887	-103	784		0		.747	8	0						1544	137	264	277	485	498
10	776	740	196	820	14	832	806	728	13	741	.319	0	-3.954	.575	9	1	14	15.499	.071	.065	.096	1544	137	300	313	162	175	-3.954
11	877	708	414	924	140	841	784	708	126	835	.212	0	-1.711	.51	21	4	2.957	3.274	.338	.305	.23	1544	137	301	314	202	215	-1.711
12	766	726	129	878	31	857	847	765	28	793		0	-2.204	.801	12	0	4.161	4.607	.24	.217		1544	137	301	314	243	256	-2.204
13	847	718	291	1013	154	866	859	776	139	915	.306	0	-1.065	.704	24	11	1.89	2.092	.529	.478	.143	1544	137	302	315	283	296	-1.065
14	757	706	326	1085	241	848	844	762	218	980	.301	0	-.583	.688	37	21	1.353	1.498	.739	.668	.243	1544	137	302	315	324	337	-.583
15	865	714	334	1102	242	888	860	777	219	995	.353	0	-.612	.704	44	25	1.38	1.528	.725	.654	.321	1544	137	303	316	364	377	-.612
16	740	720	103	861		943	933	843	-65	778	.044	0		.837	10	0					.043	1544	137	303	316	404	417
17	750	736	52	872		954	953	861	-73	788		0		.661	5	0						1544	137	304	317	445	458
18	772	757	55	888		985	978	883	-81	802		0		.815	4	0						1544	137	304	317	485	498
19	858	772	271	908	59	901	849	767	53	820	.227	0	-2.346	.4	30	4	4.593	5.085	.218	.197	.141	1544	137	340	353	162	175	-2.346
20	929	744	162	826	12	871	814	735	11	746		0	-3.902	.665	10	1	13.5	14.945	.074	.067		1544	137	341	354	202	215	-3.902

Total number of rows: 1152

Table truncated, full table size 127 Kbytes.

Supplementary data files not provided

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