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Sample GSM1720850 Query DataSets for GSM1720850
Status Public on Jun 25, 2015
Title soleus-control-6
Sample type RNA
 
Source name soleus muscle
Organism Mus musculus
Characteristics tissue: soleus
genotype: control
Sex: male
age: 40 days old
Treatment protocol Mice are homozygous for the conditional nebulin allele and are hemizygous for the muscle-specific MCK-Cre transgene (cKO) or lack the MCK-Cre transgene (Control).
Growth protocol Mice are on a mixed C57BL/6J and 1219S6/SvEvTac background. Mice are grown in a pathogen-free barrier facility with 12hr light:12hr dark cycle and are provided water and Teklad Global Rodent Diet (2919) ad libitum.
Extracted molecule total RNA
Extraction protocol Tissues were stored in Invitrogen RNAlater following manufacturer's protocol. Total RNA was isolated using Qiagen RNeasy fibrous tissue kit following the manufacturer's protocol.
Label biotin
Label protocol Samples were biotin-labelled using the Affymetrix GeneChip WT Plus reagant per manufacturer's protocol.
 
Hybridization protocol Samples were hybridized at 45C 17hours using the Affymetrix GeneChip Hybridization Buffers in with the Affymetrix Hyb Oven 645 and Fluidics Station 450.
Scan protocol The Affymetrix Scanner 3000 7G was used to scan the GeneChips using Affymetrix GeneChip Console.
Description normal soleus
Data processing Affymetrix Expression Console used RMA default settings and MoGene-1_0-st-v1.na34.mm10.transcript.csv annotation file. Affymetrix Transcript Analysis Console was used with One-Way Between-Subject ANOVA (Unpaired) and default filter criteria: Fold Change (linear) < -2 or Fold Change (linear) > 2 and ANOVA p-value (Condition pair) < 0.05.
 
Submission date Jun 24, 2015
Last update date Jun 25, 2015
Contact name Henk L Granzier
E-mail(s) granzier@email.arizona.edu
Organization name University of Arizona
Street address 1656 E Mabel St.
City Tucson
State/province AZ
ZIP/Postal code 85724
Country USA
 
Platform ID GPL6246
Series (1)
GSE70213 The effect of Nebulin-Deficiency on Skeletal Muscle

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
10344614 41.95012
10344616 2.700216
10344620 6.935474
10344622 61.56414
10344624 477.3393
10344633 399.0547
10344637 88.48137
10344653 6.686743
10344658 241.1232
10344674 5.453811
10344679 12.62236
10344707 492.2153
10344713 148.0888
10344715 5.405713
10344717 9.099863
10344719 32.92268
10344721 2.841791
10344723 34.46426
10344725 252.3557
10344741 517.5172

Total number of rows: 35557

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM1720850_ControlSoleus6.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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