|
| Status |
Public on Jun 25, 2015 |
| Title |
p53-/-_GAP940_Signal |
| Sample type |
RNA |
| |
|
| Source name |
Small intestine
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: AhCre-positive (control) P53 null
protocol: gene recombination induced via IP administration of beta-napthoflavone
tissue: small intestine
|
| Treatment protocol |
Mice treated with 3x 80mg/kg Beta-Napthoflavone in one day and culled and dissected at the desiganted day post induction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
trizol phenol extraction followed by Rneasy clean up
|
| Label |
biotin
|
| Label protocol |
PICR Std Label v 2-0 - utalizing the Enzo BioArray High Yield Transcript Labelling Kit
|
| |
|
| Hybridization protocol |
PICR Standard 11uM feature-1 protocol - carried out in the Affymetrix Hybridization Oven 640 according to the manufacturer’s instructions
|
| Scan protocol |
PICR Standard 11uM feature-1 protocol - using the Affymetrix GeneChip Scanner 3000 system with Autoloader
|
| Description |
P53 null (5 Days post Cre induction)
|
| Data processing |
Raw signal intensities were initially screened by using the MaxD/View-Program to remove false positive and false negative signals (using a cut off of at least one P value for each transcript <0.2). The data were then normalized by using the global geometric mean (GGM) normalization option within the MaxD/View-Program
Pairwise comparisons of the GGM data were then performed by SAM analysis (Statistical Analysis of Microarray)
|
| |
|
| Submission date |
Jun 25, 2015 |
| Last update date |
Jun 25, 2015 |
| Contact name |
Karen Ruth Reed |
| E-mail(s) |
reedkr@cf.ac.uk
|
| Phone |
02920688497
|
| Organization name |
ECSCRI, Cardiff University
|
| Street address |
Maindy Road
|
| City |
Cardiff |
| ZIP/Postal code |
CF24 4HQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE70262 |
The impact of P53 loss on transcriptome changes following loss of Apc in the intestine |
|
