|
Status |
Public on Feb 24, 2010 |
Title |
MSC_VUB01_1 |
Sample type |
RNA |
|
|
Source name |
Mesodermal Precursor Cells derived from hES cells line VUB01, biological replicate 1
|
Organism |
Homo sapiens |
Characteristics |
Differentiation of hES cells line VUB01 towards MPC-like cells Mesenchymal stem cells phenotype
|
Growth protocol |
Mesodermal Precursor Cells (MPC) were derived from hES by plating cells on 0,1% Gelatin coated dishes in KO-DMEM medium supplemented with 20% FBS.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from the cells using the RNeasy Mini kit (Qiagen) according to the manufacturer protocol
|
Label |
Biotin
|
Label protocol |
100 ng of total RNA was used to generate biotinylated cRNA using the GeneChip® Two-Cycle Target Labeling and Control Reagents from Affymetrix according to the manufactures protocol
|
|
|
Hybridization protocol |
Hybridisation, washing and staining procedures of the Affymetrix GeneChip® Human Genome U133 Plus 2.0 were carried out as described in the Affymetrix technical manual (see manufacturer's web site)
|
Scan protocol |
Scanning procedures of the Affymetrix GeneChip® Human Genome U133 Plus 2.0 were carried out as described in the Affymetrix technical manual (see manufacturer's web site)
|
Description |
gene expression data for VUB01 hES cell line-derived MPC
|
Data processing |
The statistical treatment of the expression data was achieved using the Array Assist 4.1 software (Stratagene). Normalization was performed using the GC-RMA algorithm.
|
|
|
Submission date |
Mar 02, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Genevieve PIETU |
E-mail(s) |
gpietu@istem.genethon.fr
|
Phone |
33169471154
|
Fax |
33169471153
|
Organization name |
INSERM
|
Department |
U861
|
Lab |
ISTEM
|
Street address |
1 rue de l'Internationale
|
City |
Evry |
ZIP/Postal code |
91000 |
Country |
France |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE7177 |
Comparison of gene expression data between wild-type and DM1-affected Mesodermal Precursors Cells (MPC) |
GSE7214 |
Comparison of gene expression data between wild-type and DM1-affected cells |
|
Relations |
Reanalyzed by |
GSE119087 |