|
Status |
Public on Oct 11, 2007 |
Title |
Neisseria meningitidis isolate A315-3 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
menA 315
|
Organism |
Neisseria meningitidis |
Characteristics |
Neisseria meningitidis Serogroup A isolate
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted by using Wizard Genomic DNA Purification Kit (Promega)
|
Label |
Cy5
|
Label protocol |
Genomic DNA was fluorescently labeled by random priming with the Klenow fragment
|
|
|
Channel 2 |
Source name |
menC 053442
|
Organism |
Neisseria meningitidis |
Characteristics |
Neisseria meningitidis Serogroup C isolate (ST4821)
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted by using Wizard Genomic DNA Purification Kit (Promega)
|
Label |
Cy3
|
Label protocol |
Genomic DNA was fluorescently labeled by random priming with the Klenow fragment
|
|
|
|
Hybridization protocol |
samples were hybridized overnight at 65°C
|
Scan protocol |
The processed slides were scanned with a GenePix 4000B scanner (Axon). Fluorescent spots and the local background intensities were quantified with GenePix Pro 5.0 software (Axon).
|
Description |
CGH
|
Data processing |
MIDAS (TIGR) GACK (Genomotyping Analysis by Charlie Kim, Genome Biology 2002)
|
|
|
Submission date |
Mar 20, 2007 |
Last update date |
Oct 10, 2007 |
Contact name |
Junping Peng |
E-mail(s) |
pengjp@gmail.com
|
Organization name |
Chinese center for disease control and prevention
|
Lab |
State key lab for molecular virology and genetic engineering
|
Street address |
No 6, Rongjing Eastern Street, BDA
|
City |
Beijing |
ZIP/Postal code |
100176 |
Country |
China |
|
|
Platform ID |
GPL5011 |
Series (1) |
GSE7331 |
CGH result of Neisseria meningitidis ST-7 isolates |
|