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Sample GSM179125 Query DataSets for GSM179125
Status Public on Apr 06, 2007
Title Animal 1 control
Sample type RNA
 
Channel 1
Source name Reference RNA
Organism synthetic construct
Characteristics Vector RNA. Hybridizes to all spots evenly
Extracted molecule total RNA
Extraction protocol RT on empty pT7T3Pac vector
Label Cy3
Label protocol indirect labeling protocol
 
Channel 2
Source name Animal 1 control
Organism Rattus norvegicus
Characteristics Strain:Sprague-Dawley, Gender:Male
Liver section removed by partial hepatectomy (t=0).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.
Label Cy5
Label protocol indirect labeling protocol
 
 
Hybridization protocol Reference and experimental probes were combined and competitively hybridized to microarrays for 18 hours at 37C.
Scan protocol Slides were scanned using ScanArray 5000 fluorescent scanner (GSI Lumonics, Billerica, MA). The resulting images were quantified using ScanArray Express v2.2 software with the Adaptive Threshold segmentation (GSI Lumonics, Billerica, MA).
Description NA
Data processing Raw quantitated array data was normalized using the print-tip lowess and scale normalization algorithms
 
Submission date Mar 31, 2007
Last update date Apr 05, 2007
Contact name Egle Juskeviciute
Organization name Thomas Jefferson University
Department Pathology
Street address 1020 Locust st. JAH 527
City Philadelphia
State/province PA
ZIP/Postal code 19107
Country USA
 
Platform ID GPL5051
Series (1)
GSE7415 Partial hepatectomy 1, 2, 4, 6h

Data table header descriptions
ID_REF
VALUE lowess normalized Cy5/Cy3

Data table
ID_REF VALUE
1 3.876454372
2 3.590559903
3 0.121191272
4 0.046912014
5 0.202053155
6 0.326922666
7 -1.082156216
8 -0.840892679
9 -1.376880877
10 -1.56358998
11 -2.00564092
12 -2.125526799
13 2.057480821
14 2.05579284
15 0.84363672
16 0.978305954
17 0.624227791
18 0.869396044
19 1.563314859
20 1.697263189

Total number of rows: 18168

Table truncated, full table size 313 Kbytes.




Supplementary file Size Download File type/resource
GSM179125.txt.gz 1.9 Mb (ftp)(http) TXT
GSM179125_Cy3_ref.tif.gz 17.8 Mb (ftp)(http) TIFF
GSM179125_Cy5_sample.tif.gz 16.7 Mb (ftp)(http) TIFF

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