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Sample GSM179159 Query DataSets for GSM179159
Status Public on Apr 06, 2007
Title Animal 10 PHx4h
Sample type RNA
 
Channel 1
Source name Reference RNA
Organism synthetic construct
Characteristics Vector RNA. Hybridizes to all spots evenly
Extracted molecule total RNA
Extraction protocol RT on empty pT7T3Pac vector
Label Cy3
Label protocol indirect labeling protocol
 
Channel 2
Source name Animal 10 PHx4h
Organism Rattus norvegicus
Characteristics Strain:Sprague-Dawley, Gender:Male
Remnat liver section removed 4 h after 70% partial hepatectomy (t=4h).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.
Label Cy5
Label protocol indirect labeling protocol
 
 
Hybridization protocol Reference and experimental probes were combined and competitively hybridized to microarrays for 18 hours at 37C.
Scan protocol Slides were scanned using ScanArray 5000 fluorescent scanner (GSI Lumonics, Billerica, MA). The resulting images were quantified using ScanArray Express v2.2 software with the Adaptive Threshold segmentation (GSI Lumonics, Billerica, MA).
Description NA
Data processing Raw quantitated array data was normalized using the print-tip lowess and scale normalization algorithms
 
Submission date Apr 01, 2007
Last update date Apr 05, 2007
Contact name Egle Juskeviciute
Organization name Thomas Jefferson University
Department Pathology
Street address 1020 Locust st. JAH 527
City Philadelphia
State/province PA
ZIP/Postal code 19107
Country USA
 
Platform ID GPL5051
Series (1)
GSE7415 Partial hepatectomy 1, 2, 4, 6h

Data table header descriptions
ID_REF
VALUE lowess normalized Cy5/Cy3

Data table
ID_REF VALUE
1 2.659739809
2 2.537976418
3 -0.79916962
4 -0.344839683
5 0.612739707
6 0.46886544
7 -1.303613147
8 -1.442974275
9 NULL
10 NULL
11 0.334286104
12 0.479366136
13 1.926496728
14 2.032194695
15 2.015075459
16 2.126523355
17 -0.733249833
18 -0.68694603
19 0.704355387
20 0.747453637

Total number of rows: 18168

Table truncated, full table size 313 Kbytes.




Supplementary file Size Download File type/resource
GSM179159.txt.gz 1.9 Mb (ftp)(http) TXT
GSM179159_Cy3_ref.tif.gz 17.1 Mb (ftp)(http) TIFF
GSM179159_Cy5_sample.tif.gz 18.6 Mb (ftp)(http) TIFF

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