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Sample GSM182113 Query DataSets for GSM182113
Status Public on Apr 17, 2007
Title p130 G0 phase Replicate 2
Sample type genomic
 
Source name p130 ChIP from G0 arrested T98G cells
Organism Homo sapiens
Characteristics human glioblastoma cell line T98G CRL-1690
Treatment protocol Cells were incubated in serum-free medium for 3 days to induce G0 arrest
Growth protocol Human T98G cells grown till confluency in DMEM supplemeted with 10 % fetal calf serum
Extracted molecule genomic DNA
Extraction protocol Chromatin was prepared from formalin-fixated cells exactly as described in Rayman, Takahashi et al. (2002) Genes Dev 16, 933-947
Label biotin
Label protocol The chromatin immunprecipitated DNA (ChIP) or 2 ng of whole genomic DNA (Input) were amplified by ligation-mediated PCR, digested with Dnase I to give fragments of ~50 bp.
The DNA fragments were end-labelled with biotin in a TdT-catalyzed reaction.
 
Hybridization protocol Affymetrix fluidics protocol EukGE-WS2v4_450
Scan protocol Scanning was carried out on an Affymetrix scanner GCS3000. The scan protocol was contained in a .cif file for chip type Hs_PromPR_v02.
Description Chromatin from G0-arrested T98G cells immunoprecipitated with anti-p130 antibody, replicate 2 out of 3
Data processing Affymetrix GCOS processed image files to generate .CEL files.
MAT (Model-based Analysis for Tiling Arrays) analysis algorithm used to detect regions enriched by chromatin immunoprecipitation (ChIP) on Affymetrix Human Promoter Array 1.0R (ChIP-chip).
 
Submission date Apr 13, 2007
Last update date Mar 09, 2012
Contact name Xiaopeng Zhu
E-mail(s) zhuxp@jimmy.harvard.edu
Organization name Institute of Biophysics, Chinese Academy of Sciences
Street address 15 Datun Street
City Beijing
ZIP/Postal code 100101
Country China
 
Platform ID GPL5082
Series (1)
GSE7516 ChIP-chip analysis of human DREAM complex subunits in G0-arrested and S-phase synchronized cells

Supplementary file Size Download File type/resource
GSM182113.CEL.gz 17.0 Mb (ftp)(http) CEL
GSM182113_CEL.txt.gz 28.6 Mb (ftp)(http) TXT
Processed data are available on Series record

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