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Sample GSM18273 Query DataSets for GSM18273
Status Public on Mar 10, 2004
Title anaerobic_arcA_rep3
Sample type RNA
 
Source name anaerobic growth of arcA knock-out strain on M9 media with glucose
Organism Escherichia coli
Extracted molecule total RNA
 
Description The purpose of this study is to investigate the changes of global gene expression in E. coli during an oxygen shift. All cultures were grown under aerobic or anaerobic conditions in M9 minimal media supplemented with glucose. Samples were RNA-stabilized using Qiagen RNAProtect Bacterial Reagent, and total RNA was isolated from exponentially growing cells using a Qiagen RNeasy mini kit (protocols available at www1.qiagen.com). The RNA (10 µg) was then used as the template for cDNA synthesis, the product of which was fragmented, labelled, and hybridized to an Affymetrix E. coli Antisense Genome Array, which was washed and scanned to obtain an image. All of these steps were performed according to Affymetrix protocols (available at www.affymetrix.com).
 
Submission date Mar 04, 2004
Last update date Aug 01, 2011
Contact name Jinseong Cheong
E-mail(s) jcheong@ucsd.edu
Phone (858)534-8758
Organization name University of California at San Diego
Street address
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platform ID GPL199
Series (2)
GSE1107 anaerobic knock-out
GSE1121 changes of global gene expression in E. coli during an oxygen shift

Data table header descriptions
ID_REF
VALUE dChip preprocessed and normalized value
ABS_CALL Affymetrix's judgement call whether a value is good or not

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 1350.6 A
AFFX-BioC-3_at 240.9 A
AFFX-CreX-5_at 10.1 A
AFFX-CreX-3_at 3.5 A
AFFX-DapX-5_at 2.9 A
AFFX-DapX-M_at 0.9 A
AFFX-DapX-3_at 1.5 A
AFFX-LysX-5_at 0.7 A
AFFX-LysX-M_at 1.9 A
AFFX-LysX-3_at 1.2 A
AFFX-PheX-5_at 2.4 A
AFFX-PheX-M_at 0.9 A
AFFX-PheX-3_at 2.9 A
AFFX-ThrX-5_at 27 A
AFFX-ThrX-M_at 4.6 A
AFFX-ThrX-3_at 57.5 A
AFFX-TrpnX-5_at 2.9 A
AFFX-TrpnX-M_at 2.3 A
AFFX-TrpnX-3_at 1 A
AFFX-HXB2_5_at 1.1 A

Total number of rows: 7312

Table truncated, full table size 194 Kbytes.




Supplementary file Size Download File type/resource
GSM18273.CEL.gz 2.2 Mb (ftp)(http) CEL

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