|
| Status |
Public on Jul 23, 2015 |
| Title |
U251 RA_#14 |
| Sample type |
RNA |
| |
|
| Source name |
glioblastoma xenografts
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U-251 glioblastoma cells xenografts
treatment: 13-cis retinoic acid
|
| Treatment protocol |
The U251 xenografts were treated daily for 18 days with 30 mg/kg 13-cis retinoic acid (RA), or 30 mg/kg Thalidomide (Thal), or by both drugs in combination (RA_Thal), dissolved in refined sesame oil and administered via intragastric tubes
|
| Growth protocol |
1.5 x 10^6 U251 human glioblastoma cells were inocculated s.c. into the right hind limb of athymic female nude mice. Palpable tumours developed within 8 to 10 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from <50 mg slices of tumours excised and snap-frozen at the end of treatment was extracted and purified using the RNeasy mini kit (Qiagen, Hilden, Germany)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
U251 human glioblastoma cells were treated with 30 mg/kg retinoic acid
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 7, from SAS. Gene expression profiling was performed using arrays of HuGene-1_0-st-type from Affymetrix. A Custom CDF Version 17 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Jul 22, 2015 |
| Last update date |
Jul 23, 2015 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17556 |
| Series (1) |
| GSE71224 |
Inhibition of 13-cis retinoic acid-induced gene expression of reactive-resistance genes by thalidomide in glioblastoma tumours in vivo |
|
