|
| Status |
Public on Jul 24, 2016 |
| Title |
Microarray-DE1 |
| Sample type |
RNA |
| |
|
| Source name |
definitive endoderm cells differentiated from hESCs
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: DE derived from hESCs
|
| Treatment protocol |
hGECs,hDECs,DEs,and GSEMFs were collected after trypsinized with TrypLE. hiMESCs were collected by manual picking.
|
| Growth protocol |
hGECs and hDECs were cultured in Kubota's medium. hiMESCs which were converted from hGECs/hDECs by small molecules were cultured in advanced DMEM/F12 with 2μM Bay K 8644, 0.5μM Bix01294 , 0.04μM RG108, 2μM SB431542. DEs were induced from hESCs in high Activin media. GSEMFs were cultured in Kubota's medium with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were prepared from hGECs,hDECs,hiMESCs, DEs,and GSEMFs using an RNeasy MiniKit (Qiagen) following the manufacturer's recommendations
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was generated from total RNA by using the Illumina TotalPrep kit (Ambion) according to the manufacturer’s instruction.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
replicate 1
|
| Data processing |
Array data were processed and analyzed by using Illumina BeadStudio software.
|
| |
|
| Submission date |
Jul 24, 2015 |
| Last update date |
Jul 24, 2016 |
| Contact name |
Jinhua Qin |
| E-mail(s) |
qinjinhuarhea@126.com
|
| Organization name |
Beijing Institute of Transfusion
|
| Street address |
Taiping Road
|
| City |
Beijing |
| ZIP/Postal code |
100850 |
| Country |
China |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE69706 |
Conversion of Human Gastric Epithelial Cells to Multipotent Endodermal Progenitors using Defined Small Molecules |
| GSE71301 |
Conversion of Human Gastric Epithelial Cells to Multipotent Endodermal Progenitors using Defined Small Molecules [array] |
|
