|
| Status |
Public on Apr 06, 2016 |
| Title |
ΔspdR x WT- rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
WT- stationary phase
|
| Organism |
Caulobacter vibrioides NA1000 |
| Characteristics |
sample type: NA1000 cells at stationary phase
genotype: WT
|
| Treatment protocol |
Cultures were grown for 24h after dilution
|
| Growth protocol |
Overnight cultures were diluted in fresh complex medium to OD= 0.1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
standard Cy3-dCTP and Cy5-dCTP incorporations standard Cy3-dCTP incorporation standard Cy3-dCTP incorporation standard Cy3-dCTP incorporation
|
| |
|
| Channel 2 |
| Source name |
ΔspdR- stationary phase
|
| Organism |
Caulobacter vibrioides NA1000 |
| Characteristics |
genotype: ΔspdR
sample type: spdR mutant at stationary phase
|
| Treatment protocol |
Cultures were grown for 24h after dilution
|
| Growth protocol |
Overnight cultures were diluted in fresh complex medium to OD= 0.1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
standard Cy3-dCTP and Cy5-dCTP incorporations standard Cy3-dCTP incorporation standard Cy3-dCTP incorporation standard Cy3-dCTP incorporation
|
| |
|
| |
| Hybridization protocol |
Agilent protocol Agilent protocol Agilent protocol Agilent protocol
|
| Scan protocol |
Agilent protocol Agilent protocol
|
| Data processing |
Data were extracted and normalized using Agilent Feature Extraction Software 9.0. Agilent output files were then opened using Excell and the log10 values extracted with no additional processing or filtering
|
| |
|
| Submission date |
Jul 24, 2015 |
| Last update date |
Apr 07, 2016 |
| Contact name |
Rogerio Lourenco |
| E-mail(s) |
lourenco@iq.usp.br
|
| Organization name |
Universidade de Sao Paulo
|
| Department |
Bioquimica
|
| Street address |
Av. Prof. Lineu Prestes, 748
|
| City |
Sao Paulo |
| State/province |
Sao Paulo |
| ZIP/Postal code |
05508-900 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL10469 |
| Series (1) |
|
