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Sample GSM1834396 Query DataSets for GSM1834396
Status Public on Jun 23, 2016
Title RH-RAS LSC, line 3, treated with Dot1l inhibitor
Sample type RNA
 
Source name transformed leukemia stem cells
Organism Mus musculus
Characteristics cell line: RH-RAS LSC
treatment: Dot1l inhibitor
Treatment protocol Total RNA was isolated with the Rneasy Plus Miniprep Kit (Qiagen).
Growth protocol RH-RAS and other Leukemia stem cell (LSC) lines were cultured in Opti-MEM base medium supplemented with 10% FBS, 1% antibiotics, 50 _M mercaptoethanol and stem cell factor
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA and further RNeasy Kit purification was performed according to the manufacturer's instructions.
Label biotin
Label protocol Standard Affymetrix protocol
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description gene expression data from Dot1l inhibitor treated RH-RAS LSCs
Data processing Hybridization signals were retrieved and normalized by RMA
 
Submission date Jul 28, 2015
Last update date Jun 23, 2016
Contact name Rui Lu
E-mail(s) ruilu1@uabmc.edu
Organization name Univ of Alabama at Birmingham
Street address 1824 6th Ave S
City Birmingham
State/province AL
ZIP/Postal code 35294
Country USA
 
Platform ID GPL1261
Series (2)
GSE71439 Expression profiling of murine leukemia stem cell (LSC) lines established ex vivo by coexpression of R882H-mutated DNMT3A and NRAS-G12D post treatment with Dot1l inhibitor (Microarray)
GSE71475 Epigenetic perturbations by Arg882-mutated DNMT3A potentiate aberrant stem cell gene expression program and acute leukemia development

Data table header descriptions
ID_REF
VALUE RMA normailized values, log2 transformed

Data table
ID_REF VALUE
1415670_at 9.599986052
1415671_at 11.76790874
1415672_at 12.10591951
1415673_at 10.72758683
1415674_a_at 10.45146575
1415675_at 9.681981965
1415676_a_at 12.32445805
1415677_at 9.615397619
1415678_at 10.60245359
1415679_at 11.93475755
1415680_at 10.76819582
1415681_at 10.9820741
1415682_at 9.576864768
1415683_at 11.79376262
1415684_at 9.336589849
1415685_at 9.46250968
1415686_at 10.80068392
1415687_a_at 13.50316376
1415688_at 10.8148242
1415689_s_at 9.263072531

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM1834396_RH-RAS-3-SGC.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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