|
| Status |
Public on Jun 23, 2016 |
| Title |
MLL-AF9 LSC |
| Sample type |
RNA |
| |
|
| Source name |
transformed leukemia stem cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: MLL-AF9 LSC
|
| Treatment protocol |
Total RNA was isolated with the Rneasy Plus Miniprep Kit (Qiagen).
|
| Growth protocol |
RH-RAS and other Leukemia stem cell (LSC) lines were cultured in Opti-MEM base medium supplemented with 10% FBS, 1% antibiotics, 50 _M mercaptoethanol and stem cell factor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and further RNeasy Kit purification was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
gene expression data from MLL-AF9 LSCs
|
| Data processing |
Hybridization signals were retrieved and normalized by RMA
|
| |
|
| Submission date |
Jul 28, 2015 |
| Last update date |
Jun 23, 2016 |
| Contact name |
Rui Lu |
| E-mail(s) |
ruilu1@uabmc.edu
|
| Organization name |
Univ of Alabama at Birmingham
|
| Street address |
1824 6th Ave S
|
| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35294 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE71439 |
Expression profiling of murine leukemia stem cell (LSC) lines established ex vivo by coexpression of R882H-mutated DNMT3A and NRAS-G12D post treatment with Dot1l inhibitor (Microarray) |
| GSE71475 |
Epigenetic perturbations by Arg882-mutated DNMT3A potentiate aberrant stem cell gene expression program and acute leukemia development |
|
