|
| Status |
Public on Aug 01, 2018 |
| Title |
COLO741 ABCB5-negative cells |
| Sample type |
RNA |
| |
|
| Source name |
COLO741 ABCB5-negative cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: COLO741
cell type: colon carcinoma
|
| Treatment protocol |
ABCB5-positive cells and ABCB5-negative cell populations were isolated by FACS cell sorting using anti-ABCB5 mAb described (Ksander et al., Nature, 2014).
|
| Growth protocol |
Cells were cultured in RPMI 1640 medium (Lonza Bio-Whittaker) supplemented with 10% (v/v) FBS (Invitrogen GIBCO) and 1% (v/v) penicillin/streptomycin (Lonza Bio-Whittaker).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNAeasy kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was processed using the Ambion WT Expression Kit (cat # 4425209) followed by labeling with Affymetrix GeneChip WT.
|
| |
|
| Hybridization protocol |
Terminal labeling RNA labeling and hybridization (cat # 702808) was done onto Affymetrix Human Gene 1.0ST arrays.The arrays were washed and stained on Fluidics Stations 450 using the Affymetrix Hybe/Wash/Stain (cat # 900720).
|
| Scan protocol |
The arrays were scanned on GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from ABCB5-negative COLO741 cells
|
| Data processing |
The CHP files were generated from the CEL files using Affymetrix Expression Console Software.
|
| |
|
| Submission date |
Aug 03, 2015 |
| Last update date |
Aug 01, 2018 |
| Contact name |
Natasha Y Frank |
| E-mail(s) |
nfrank@partners.org
|
| Phone |
617 919 4882
|
| Organization name |
Children's Hospital Boston
|
| Department |
Medicine
|
| Street address |
320 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE71670 |
The stem cell gene ABCB5 mediates cancer resistance to apoptosis |
|
