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Sample GSM1842684 Query DataSets for GSM1842684
Status Public on Aug 01, 2018
Title COLO741 ABCB5-positive cells
Sample type RNA
 
Source name COLO741 ABCB5-positive cells
Organism Homo sapiens
Characteristics cell line: COLO741
cell type: colon carcinoma
Treatment protocol ABCB5-positive cells and ABCB5-negative cell populations were isolated by FACS cell sorting using anti-ABCB5 mAb described (Ksander et al., Nature, 2014).
Growth protocol Cells were cultured in RPMI 1640 medium (Lonza Bio-Whittaker) supplemented with 10% (v/v) FBS (Invitrogen GIBCO) and 1% (v/v) penicillin/streptomycin (Lonza Bio-Whittaker).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using RNAeasy kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA was processed using the Ambion WT Expression Kit (cat # 4425209) followed by labeling with Affymetrix GeneChip WT.
 
Hybridization protocol Terminal labeling RNA labeling and hybridization (cat # 702808) was done onto Affymetrix Human Gene 1.0ST arrays.The arrays were washed and stained on Fluidics Stations 450 using the Affymetrix Hybe/Wash/Stain (cat # 900720).
Scan protocol The arrays were scanned on GeneChip Scanner 3000 7G.
Description Gene expression data from ABCB5-positive COLO741 cells
Data processing The CHP files were generated from the CEL files using Affymetrix Expression Console Software.
 
Submission date Aug 03, 2015
Last update date Aug 01, 2018
Contact name Natasha Y Frank
E-mail(s) nfrank@partners.org
Phone 617 919 4882
Organization name Children's Hospital Boston
Department Medicine
Street address 320 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6244
Series (1)
GSE71670 The stem cell gene ABCB5 mediates cancer resistance to apoptosis

Data table header descriptions
ID_REF
VALUE RMA Signal

Data table
ID_REF VALUE
7892501 6.76618
7892502 7.11201
7892503 4.87372
7892504 9.98076
7892505 5.2198
7892506 7.20585
7892507 7.24581
7892508 8.56913
7892509 14.0667
7892510 5.93103
7892511 6.01514
7892512 8.43256
7892513 4.98729
7892514 13.326
7892515 11.1034
7892516 6.21351
7892517 8.45994
7892518 5.7649
7892519 8.42449
7892520 10.9103

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM1842684_COLO741_ABCB5-positive_cells.CEL.gz 5.1 Mb (ftp)(http) CEL
GSM1842684_COLO741_ABCB5-positive_cells.chp.gz 254.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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