|
Status |
Public on Aug 06, 2016 |
Title |
ΔphoU;Pvan-phoU -vanillate/+vanillate 7 hours depletion rep 2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
mixed population in PYE + vanillate
|
Organism |
Caulobacter vibrioides NA1000 |
Characteristics |
genotype: ΔphoU;Pvan-phoU treatment: 50 mM vanillate
|
Growth protocol |
Cells were grown to mid-log phase in rich medium (PYE) supplemented with 50 mM vanillate, washed, and released into PYE with or without vanillate. Cells were grown in mid-log phase for the specified number of hours before sampling.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNAeasy kit (Qiagen) according to manufacturer's protoco
|
Label |
Cy3
|
Label protocol |
RNA was reverse transcribed into cDNA as described previously (Laub et al. 2000)
|
|
|
Channel 2 |
Source name |
mixed population in PYE - vanillate
|
Organism |
Caulobacter vibrioides NA1000 |
Characteristics |
genotype: ΔphoU;Pvan-phoU treatment: without vanillate
|
Growth protocol |
Cells were grown to mid-log phase in rich medium (PYE) supplemented with 50 mM vanillate, washed, and released into PYE with or without vanillate. Cells were grown in mid-log phase for the specified number of hours before sampling.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNAeasy kit (Qiagen) according to manufacturer's protoco
|
Label |
Cy5
|
Label protocol |
RNA was reverse transcribed into cDNA as described previously (Laub et al. 2000)
|
|
|
|
Hybridization protocol |
Agilent protocol
|
Scan protocol |
Arrays were scanned using Agilent Technologies Scanner G2505C Arrays were scanned using Agilent Technologies Scanner G2505C
|
Description |
Cells grown in PYE + vanillate to mid-log phase, washed, and released into PYE + or - vanillate and grown in mid-log phase for the specified number of hours
|
Data processing |
Data were retrieved using Agilent Feature Extractor 10.6
|
|
|
Submission date |
Aug 07, 2015 |
Last update date |
Aug 06, 2016 |
Contact name |
Michael Laub |
Organization name |
MIT
|
Street address |
31 Ames St
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL15487 |
Series (2) |
GSE71857 |
Identification of the PhoB regulon and role of PhoU in the phosphate-starvation response of Caulobacter crescentus (expression) |
GSE71961 |
Identification of the PhoB regulon and role of PhoU in the phosphate-starvation response of Caulobacter crescentus |
|