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Sample GSM1872256 Query DataSets for GSM1872256
Status Public on Sep 15, 2015
Title dicerwt_R1 Replicate 1
Sample type RNA
Source name RKO dicer wild-type cells
Organism Homo sapiens
Characteristics genotype/variation: dicer wt
transgene: cells expressing wild-type RNase1
Growth protocol Cells were cultured in serum-free PC-1 media (Lonza).
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol followed by two additional round of phenol-chloroform extraction.
Label Cy3,Cy5
Label protocol 750 ng total RNA from both sample and reference was labeled with Hy3 and Hy5 fluorescent label, respectively, using the miRCURY LNA miRNA Hi-Power Labeling kit, Hy3/Hy5 (Exiqon,Denmark) following the procedure described by the manufaturer.
Hybridization protocol The hybridization was performed according to the miRCURY LNA miRNA Array Instruction manual using a Tecan HS4800 hybridization station.
Scan protocol The miRCURY LNA micro RNA Array slides were scanned using the Agilent G2565 BA Microarray Scanner System.
Data processing The quantified signals were background corrected (Normexp with offset value 10) and normalized using the global Lowess regression algorithm. The normalized expression levels are expressed in a log2 scale.
Submission date Sep 08, 2015
Last update date Sep 15, 2015
Contact name Emanuele Canestrari
Organization name University of Illinois at Chicago
Street address 900 S. Ashland Ave.(M/C 669)
City Chicago
ZIP/Postal code 60607
Country USA
Platform ID GPL20891
Series (1)
GSE72796 Rnase1 responsive miRNA

Data table header descriptions
VALUE lowess log2 values

Data table
4040 5.259612262
4610 7.377058894
4700 6.64557707
5250 4.333664826
6880 5.662557919
9938 9.930042833
10138 5.517445578
10306 5.316551029
10916 5.123031481
10919 9.966340363
10923 9.176828873
10925 6.732822771
10928 8.69267582
10936 7.453007995
10937 5.380515651
10943 5.248032845
10946 6.106942626
10947 5.318520114
10952 5.116039427
10954 5.421490904

Total number of rows: 2100

Table truncated, full table size 36 Kbytes.

Supplementary file Size Download File type/resource
GSM1872256_Cy3_Exiqon_19735992_S01.txt.gz 1.5 Mb (ftp)(http) TXT
GSM1872256_Cy5_Exiqon_19735992_S01.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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