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Sample GSM1872259 Query DataSets for GSM1872259
Status Public on Sep 15, 2015
Title dicerwt_6x Replicate 2
Sample type RNA
Source name RKO dicer wild-type cells
Organism Homo sapiens
Characteristics genotype/variation: dicer wt
transgene: cells expressing mutant RNase1
Growth protocol Cells were cultured in serum-free PC-1 media (Lonza).
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol followed by two additional round of phenol-chloroform extraction.
Label Cy3,Cy5
Label protocol 750 ng total RNA from both sample and reference was labeled with Hy3 and Hy5 fluorescent label, respectively, using the miRCURY LNA miRNA Hi-Power Labeling kit, Hy3/Hy5 (Exiqon,Denmark) following the procedure described by the manufaturer.
Hybridization protocol The hybridization was performed according to the miRCURY LNA miRNA Array Instruction manual using a Tecan HS4800 hybridization station.
Scan protocol The miRCURY LNA micro RNA Array slides were scanned using the Agilent G2565 BA Microarray Scanner System.
Data processing The quantified signals were background corrected (Normexp with offset value 10) and normalized using the global Lowess regression algorithm. The normalized expression levels are expressed in a log2 scale.
Submission date Sep 08, 2015
Last update date Sep 15, 2015
Contact name Emanuele Canestrari
Organization name University of Illinois at Chicago
Street address 900 S. Ashland Ave.(M/C 669)
City Chicago
ZIP/Postal code 60607
Country USA
Platform ID GPL20891
Series (1)
GSE72796 Rnase1 responsive miRNA

Data table header descriptions
VALUE lowess log2 values

Data table
4040 5.655994404
4610 7.575319102
4700 6.69015705
5250 5.654729641
6880 5.993775794
9938 9.971962864
10138 5.930167705
10306 5.707672099
10916 5.674905407
10919 9.961072124
10923 9.099234509
10925 6.838774543
10928 8.614304555
10936 7.516550027
10937 5.809769783
10943 5.706370526
10946 6.383028865
10947 5.697550156
10952 5.551900144
10954 5.832106706

Total number of rows: 2100

Table truncated, full table size 37 Kbytes.

Supplementary file Size Download File type/resource
GSM1872259_Cy3_Exiqon_19735942_S01.txt.gz 1.6 Mb (ftp)(http) TXT
GSM1872259_Cy5_Exiqon_19735942_S01.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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