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Sample GSM1880771 Query DataSets for GSM1880771
Status Public on Sep 15, 2015
Title Ly49- CD8 cells, rep1
Sample type RNA
Source name Ly49- CD8 T cells
Organism Mus musculus
Characteristics strain/background: B6
genotype/variation: wild-type
tissue derivation: spleen
cell type: conventional memory type of CD8+ T cells (CD44+CD122+Ly49-)
Treatment protocol n.a.
Growth protocol n.a.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from sorted CD8 T cells using RNeasy kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization was done according to the Affymetrix 3' IVT Express protocol.
Scan protocol The arrays were scanned on the GeneChip Scanner 3000 7G.
Description HC2010110802_Ly49-1
Gene expression data from Ly49- CD8 T cells.
Data processing Data were analyzed using the GenePattern software package obtained from the Broad Institute. The raw microarray data were normalized using Expression FileCreater and analyzed using the MultiPlot module in GenePattern.
Submission date Sep 14, 2015
Last update date Sep 15, 2015
Contact name Hye-Jung Kim
Organization name Dana Farber Cancer Institute
Department Cancer Immunology and Virology
Lab Cantor
Street address 450 Brookline
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platform ID GPL1261
Series (1)
GSE73015 Stable inhibitory activity of regulatory T cells requires the transcription factor Helios

Data table header descriptions
VALUE RMA method

Data table
1415670_at 4033.281088
1415671_at 3073.831347
1415672_at 5184.576158
1415673_at 2812.929893
1415674_a_at 3443.691185
1415675_at 5409.437113
1415676_a_at 10065.06513
1415677_at 1977.586228
1415678_at 11127.39522
1415679_at 9355.190317
1415680_at 4572.175347
1415681_at 3680.216448
1415682_at 2397.777819
1415683_at 5418.065135
1415684_at 1689.898468
1415685_at 2792.72155
1415686_at 5368.797456
1415687_a_at 10099.14309
1415688_at 5625.180737
1415689_s_at 1975.716665

Total number of rows: 45101

Table truncated, full table size 1025 Kbytes.

Supplementary file Size Download File type/resource
GSM1880771_HC2010110802_Ly49-1.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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