NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1880773 Query DataSets for GSM1880773
Status Public on Sep 15, 2015
Title Ly49- CD8 cells, rep2
Sample type RNA
 
Source name Ly49- CD8 T cells
Organism Mus musculus
Characteristics strain/background: B6
genotype/variation: wild-type
tissue derivation: spleen
cell type: conventional memory type of CD8+ T cells (CD44+CD122+Ly49-)
Treatment protocol n.a.
Growth protocol n.a.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from sorted CD8 T cells using RNeasy kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization was done according to the Affymetrix 3' IVT Express protocol.
Scan protocol The arrays were scanned on the GeneChip Scanner 3000 7G.
Description HC2010110805_Ly49-2
Gene expression data from Ly49- CD8 T cells.
Data processing Data were analyzed using the GenePattern software package obtained from the Broad Institute. The raw microarray data were normalized using Expression FileCreater and analyzed using the MultiPlot module in GenePattern.
 
Submission date Sep 14, 2015
Last update date Sep 15, 2015
Contact name Hye-Jung Kim
Organization name Dana Farber Cancer Institute
Department Cancer Immunology and Virology
Lab Cantor
Street address 450 Brookline
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE73015 Stable inhibitory activity of regulatory T cells requires the transcription factor Helios

Data table header descriptions
ID_REF
VALUE RMA method

Data table
ID_REF VALUE
1415670_at 4289.504176
1415671_at 3150.815135
1415672_at 4727.601667
1415673_at 2889.10943
1415674_a_at 3570.815135
1415675_at 5628.681344
1415676_a_at 10192.14949
1415677_at 2072.853685
1415678_at 10511.83866
1415679_at 9356.34723
1415680_at 4662.731952
1415681_at 3854.831199
1415682_at 2464.973549
1415683_at 5548.129052
1415684_at 1566.260844
1415685_at 2740.933134
1415686_at 5439.759105
1415687_a_at 10910.93589
1415688_at 5093.992171
1415689_s_at 2236.712296

Total number of rows: 45101

Table truncated, full table size 1025 Kbytes.




Supplementary file Size Download File type/resource
GSM1880773_HC2010110805_Ly49-2.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap