|
| Status |
Public on Oct 01, 2015 |
| Title |
AF [12DNJ007] |
| Sample type |
genomic |
| |
|
| Source name |
Prenatal diagnosis_AF
|
| Organism |
Homo sapiens |
| Characteristics |
sample origin: Prenatal diagnosis
clinical indication: holoprosencephaly, facial deformity (cyclopia,beak nose)
tissue: Amniotic fluid
|
| Treatment protocol |
Samples were untreated
|
| Growth protocol |
Uncultured samples of chorionic villus sampling, amniotic fluid, placental tissue and blood were used for direct DNA extraction
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA extraction done by Qiagen DNeasy® Blood & Tissue Kit following manufacturer's instructions
|
| Label |
C-Bio and A-DNP
|
| Label protocol |
Illumina standard protocol for CytoSNP 12 chips (GPL16483), using automation with the Tecan Evo robot.
|
| |
|
| Hybridization protocol |
Illumina standard protocol for CytoSNP 12 chips (GPL16483), using automation with the Tecan Evo robot.
|
| Scan protocol |
Illumina standard protocol for CytoSNP 12 chips (GPL16483), using automation with the Tecan Evo robot.
|
| Data processing |
Genomic DNA was genotyped using KaryoStudio V 1.3.11 and GenomeStudio V2011.1 (Illumina) based on hg19.
|
| |
|
| Submission date |
Sep 18, 2015 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Zirui DONG |
| E-mail(s) |
elvisdong@link.cuhk.edu.hk
|
| Organization name |
The Chinese University of Hong Kong
|
| Department |
Dept. of Obstetrics and Gynaecology
|
| Street address |
Shatin, N.T.
|
| City |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
China |
| |
|
| Platform ID |
GPL8855 |
| Series (2) |
| GSE73190 |
Chromosomal microarray analysis for validation of the NGS-based CNV detection results in clinical samples |
| GSE73191 |
Chromosomal microarray data for validation of copy-number variants detection from a low-coverage whole-genome sequencing approach in clinical samples |
|
