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Status |
Public on Sep 01, 2009 |
Title |
REF52neoTAg, bio rep 2 |
Sample type |
RNA |
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Source name |
REF52neoTAg3, 2 day post-confluence
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Organism |
Rattus norvegicus |
Characteristics |
REF52neo cells stably expressing SV40 Large T antigen gene (Clone 3)
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Treatment protocol |
Cells were grown to confluence and fed with fresh medium. After two days, cells were harvested with trypsin, washed 3X with PBS-EDTA and cell pellet was frozen until RNA isolation.
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Growth protocol |
MEM + 10% Fetal Bovine Serum + 400ug/ml G418 + Penn/Strep
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Extracted molecule |
polyA RNA |
Extraction protocol |
Total RNA was isolated using the Ultraspec II RNA reagent (Biotex). Poly-A+ RNA was isolated from the total RNA using the Oligotex kit (Qiagen).
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Label |
biotin
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Label protocol |
Standard Affymetrix labeling protocol done by a Core Facility
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Hybridization protocol |
Standard Affymetrix hybridization protocol done by a Core Facility
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Scan protocol |
Standard Affymetrix scan protocol done by a Core Facility
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Description |
Center for Applied Genomics (Newark, NJ)
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Data processing |
Microarray Suite Version 5.0 (MAS 5.0) generated .CEL files were converted into RMA expression values using the BRB-Array Tools software (an Excel plug-in developed by Richard Simon at NCI - http://linus.nci.nih.gov/BRB-ArrayTools.html). Affymetrix Present/Absent calls and intensity values were calculated with MAS 5.0 using Affymetrix default analysis settings and global scaling with target signal = 500 as the normalization method.
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Submission date |
May 17, 2007 |
Last update date |
May 29, 2009 |
Contact name |
Daniel Clark |
Organization name |
University of Pittsburgh
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Street address |
3501 Terrace St.
|
City |
Pittsburgh |
ZIP/Postal code |
15213 |
Country |
USA |
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|
Platform ID |
GPL85 |
Series (2) |
GSE7836 |
Global analysis of gene expression in REF52 cells expressing SV40 T antigens |
GSE7906 |
Cell-type Specific Regulation of Gene Expression by Simian Virus 40 T antigens |
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