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Sample GSM190556 Query DataSets for GSM190556
Status Public on Jul 27, 2009
Title Fat CD4 Treg cells, repl. 2
Sample type RNA
 
Source name Abdominal adipose tissue Treg cells
Organism Mus musculus
Characteristics Male C57Bl/6 (retired breeders 25-35 weeks old) purchased from the Jackson Laboratory. Abdominal (epidydimal) adipose tissue was cut into small pieces and digested for about 40 minutes with collagease type II. Cell suspensions were then filtered through a sieve and stromovascular fraction (SVF) was harvested after spinning.
Genetic background: Male C57Bl/6;
Age: 25-35 weeks old;
Number of mice per chip: 8-10;
Tissue: Abdominal (epidydimal) adipose tissue;
Sorting: B220-, CD11b-, CD8a-, CD4+, TCRb+, CD25+
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3507.CEL
Data processing RMA
 
Submission date May 18, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7852 Fat Treg cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 208.9005492
1415671_at 533.5757481
1415672_at 2080.37136
1415673_at 186.6976702
1415674_a_at 342.1917312
1415675_at 336.6901646
1415676_a_at 879.8161613
1415677_at 171.5828038
1415678_at 1309.932459
1415679_at 3970.71797
1415680_at 367.8833665
1415681_at 299.8216081
1415682_at 207.4125505
1415683_at 1264.876219
1415684_at 199.4532927
1415685_at 179.4331109
1415686_at 984.9116261
1415687_a_at 1234.151916
1415688_at 2182.515789
1415689_s_at 238.5252584

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM190556.CEL.gz 3.7 Mb (ftp)(http) CEL

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