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Sample GSM190563 Query DataSets for GSM190563
Status Public on Jul 27, 2009
Title Fat CD4 Tconv cells, repl. 1
Sample type RNA
Source name Abdominal adipose tissue Tconv
Organism Mus musculus
Characteristics Male C57Bl/6 (retired breeders 25-35 weeks old) purchased from the Jackson Laboratory. Abdominal (epidydimal) adipose tissue was cut into small pieces and digested for about 40 minutes with collagease type II. Cell suspensions were then filtered through a sieve and stromovascular fraction (SVF) was harvested after spinning.
Genetic background: Male C57Bl/6;
Age: 25-35 weeks old;
Number of mice per chip: 8-10;
Tissue: Abdominal (epidydimal) adipose tissue;
Sorting: B220-, CD11b-, CD8a-, CD4+, TCRb+, CD25-
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3643.CEL
Data processing RMA
Submission date May 18, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platform ID GPL1261
Series (1)
GSE7852 Fat Treg cells
Reanalyzed by GSE119085

Data table header descriptions
VALUE RMA-unlogged

Data table
1415670_at 134.3325462
1415671_at 384.8945662
1415672_at 2594.432356
1415673_at 140.4844273
1415674_a_at 163.5782928
1415675_at 210.9949594
1415676_a_at 628.5366261
1415677_at 97.0149283
1415678_at 1056.858656
1415679_at 3388.232258
1415680_at 265.655618
1415681_at 278.0376277
1415682_at 192.3831486
1415683_at 1220.555346
1415684_at 170.4967393
1415685_at 117.4030286
1415686_at 1501.799
1415687_a_at 1397.063178
1415688_at 2667.836628
1415689_s_at 160.361747

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM190563.CEL.gz 3.5 Mb (ftp)(http) CEL

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