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Sample GSM190564 Query DataSets for GSM190564
Status Public on Jul 27, 2009
Title Fat CD4 Tconv cells, repl. 2
Sample type RNA
Source name Abdominal adipose tissue Tconv cells
Organism Mus musculus
Characteristics Male C57Bl/6 (retired breeders 25-35 weeks old) purchased from the Jackson Laboratory. Abdominal (epidydimal) adipose tissue was cut into small pieces and digested for about 40 minutes with collagease type II. Cell suspensions were then filtered through a sieve and stromovascular fraction (SVF) was harvested after spinning.
Genetic background: Male C57Bl/6;
Age: 25-35 weeks old;
Number of mice per chip: 8-10;
Tissue: Abdominal (epidydimal) adipose tissue;
Sorting: B220-, CD11b-, CD8a-, CD4+, TCRb+, CD25-
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3508.CEL
Data processing RMA
Submission date May 18, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platform ID GPL1261
Series (1)
GSE7852 Fat Treg cells
Reanalyzed by GSE119085

Data table header descriptions
VALUE RMA-unlogged

Data table
1415670_at 228.7457222
1415671_at 408.9233809
1415672_at 1870.385997
1415673_at 272.9917511
1415674_a_at 268.8904004
1415675_at 283.7710605
1415676_a_at 802.8419007
1415677_at 129.2393223
1415678_at 1298.515793
1415679_at 3464.895074
1415680_at 459.2152323
1415681_at 525.6722593
1415682_at 254.4078549
1415683_at 1486.12697
1415684_at 254.5732897
1415685_at 275.5157927
1415686_at 972.6196613
1415687_a_at 1508.425262
1415688_at 2491.042003
1415689_s_at 372.1337973

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM190564.CEL.gz 3.5 Mb (ftp)(http) CEL

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