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Sample GSM1908040 Query DataSets for GSM1908040
Status Public on Jun 07, 2023
Title Cybrid1_J2
Sample type SRA
Source name Osteosarcoma
Organism Homo sapiens
Characteristics cell line: 143B
type of tissue: Tumour cell line
cell type: Osteosarcoma 143B
mitochondrial haplogroup: J2
Treatment protocol Cold PBS wash. Twice.
Growth protocol Growth with DMEM. Subconfluent cells were tripsinised.
Extracted molecule polyA RNA
Extraction protocol RNA isolation kit (mirVana™ miRNA Isolation Kit)
Illumina TruSeq mRNA library preparation
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description 106J
Data processing Data filtering: seqtk 1.0; Trim-galore 0.3.5; Perl script to remove poly-N tails
Mapping: tophat-2.0.6.Linux_x86_64
Counting reads mapped to genes: BEDTools 2.17.0
Testing for differentially expressed genes: DESeq2 1.0.19
Genome_build: hg19
Supplementary_files_format_and_content: Tab-delimited text files Gene count file
Submission date Oct 14, 2015
Last update date Jun 07, 2023
Contact name Aurora Gomez Duran
Phone 696876193
Organization name Centro de Investigaciones Biologicas
Department Molecular Biomedicine
Lab MitoPhenomics
Street address Maria maeztu
City Madrid
State/province Madrid
ZIP/Postal code 28040
Country Spain
Platform ID GPL11154
Series (1)
GSE74009 Mitochondrial transfer disrupts mTORC1/Akt signaling modulating biogenesis and respiration
BioSample SAMN04166256
SRA SRX1334847

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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