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Sample GSM1914636 Query DataSets for GSM1914636
Status Public on Dec 21, 2015
Title 1
Sample type RNA
Source name Primary human osteoblasts
Organism Homo sapiens
Characteristics cy3 data file: 1_Exiqon_19708476_S01.txt
cy5 data file: 0_Exiqon_19708476_S01.txt
tissue: Primary bone cells
condition: Osteoarthritis
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using High Pure RNA Isolation kit (Roche Diagnostics, Indianapolis, USA)
Label biotin
Label protocol 750 ng total RNA from both sample and reference was labeled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ (Exiqon, Denmark) following the procedure described by the manufacturer
Hybridization protocol The Hy3™-labeled samples and a Hy5™-labeled reference RNA sample were mixed pair-wise and hybridized to the miRCURY LNA™ microRNA Array 7th gen (Exiqon, Denmark). The hybridization was performed according to the miRCURY LNA™ microRNA Array Instruction manual using a Tecan HS4800™ hybridization station (Tecan, Austria).
Scan protocol microarray slides were scanned and stored in an ozone free environment (ozone level below 2.0 ppb) using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA)
Description Primary osteoblasts isolated from bone tissue
Data processing The quantified signals were background corrected (Normexp with offset value 10, see Ritchie et al. 2007) and normalized using the global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm.
Submission date Oct 20, 2015
Last update date Oct 19, 2023
Contact name Júlia Perera-Bel
Organization name Hospital del Mar Research Institute
Department MARData-BU
Street address Doctor Aiguader, 88
City Barcelona
ZIP/Postal code 08003
Country Spain
Platform ID GPL20999
Series (1)
GSE74211 Primary Human Osteoblasts miRNA array

Data table header descriptions
VALUE Normalized data

Data table
2 -0.229
3 -0.209
4 -0.853
5 -0.310
6 0.159
9 -0.469
10 0.468
13 0.366
14 0.278
15 0.532
16 0.258
17 0.543
18 0.322
19 0.220
20 0.145
21 -0.091
22 -0.173
23 0.602
24 -0.301
25 -0.288

Total number of rows: 2068

Table truncated, full table size 22 Kbytes.

Supplementary file Size Download File type/resource
GSM1914636_0_Exiqon_19708476_S01.txt.gz 1.2 Mb (ftp)(http) TXT
GSM1914636_1_Exiqon_19708476_S01.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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