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Sample GSM1914637 Query DataSets for GSM1914637
Status Public on Dec 21, 2015
Title 2b
Sample type RNA
Source name Primary human osteoblasts
Organism Homo sapiens
Characteristics cy3 data file: 1_Exiqon_19708489_S01.txt
cy5 data file: 0_Exiqon_19708489_S01.txt
tissue: Primary bone cells
condition: Osteoarthritis
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using High Pure RNA Isolation kit (Roche Diagnostics, Indianapolis, USA)
Label biotin
Label protocol 750 ng total RNA from both sample and reference was labeled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY LNA™ microRNA Hi-Power Labeling Kit, Hy3™/Hy5™ (Exiqon, Denmark) following the procedure described by the manufacturer
Hybridization protocol The Hy3™-labeled samples and a Hy5™-labeled reference RNA sample were mixed pair-wise and hybridized to the miRCURY LNA™ microRNA Array 7th gen (Exiqon, Denmark). The hybridization was performed according to the miRCURY LNA™ microRNA Array Instruction manual using a Tecan HS4800™ hybridization station (Tecan, Austria).
Scan protocol microarray slides were scanned and stored in an ozone free environment (ozone level below 2.0 ppb) using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA)
Description Primary osteoblasts isolated from bone tissue
Data processing The quantified signals were background corrected (Normexp with offset value 10, see Ritchie et al. 2007) and normalized using the global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm.
Submission date Oct 20, 2015
Last update date Oct 19, 2023
Contact name Júlia Perera-Bel
Organization name Hospital del Mar Research Institute
Department MARData-BU
Street address Doctor Aiguader, 88
City Barcelona
ZIP/Postal code 08003
Country Spain
Platform ID GPL20999
Series (1)
GSE74211 Primary Human Osteoblasts miRNA array

Data table header descriptions
VALUE Normalized data

Data table
2 -0.221
3 0.213
4 -0.932
5 -0.421
6 0.381
9 -0.698
10 0.439
13 0.389
14 0.458
15 0.373
16 -0.497
17 0.494
18 0.662
19 0.186
20 0.298
21 0.007
22 -0.356
23 0.805
24 -0.554
25 -0.384

Total number of rows: 2068

Table truncated, full table size 22 Kbytes.

Supplementary file Size Download File type/resource
GSM1914637_0_Exiqon_19708489_S01.txt.gz 1.2 Mb (ftp)(http) TXT
GSM1914637_1_Exiqon_19708489_S01.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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