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Sample GSM1915105 Query DataSets for GSM1915105
Status Public on Feb 01, 2016
Title day0_rep1
Sample type RNA
Source name mouse ES cells with SFEBq
Organism Mus musculus
Characteristics cell type: differentiated mouse embryonic stem cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using QIAcubu’s RNA micro extraction mode (Qiagen), and cDNA was synthesized using SuperScript II Reverse Transcriptase (invitrogen).
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
Hybridization protocol RNA was subsequently fragmented and hybridized to the GeneChip according to manufacturer’s instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix).
Description Day0 sample (ES cells) from differentiated mouse ES cells with SFEBq, duplicate1
Data processing The raw data were summarized with the RMA algorithm (R 2.13.0, Bioconductor 2.8).
Submission date Oct 21, 2015
Last update date Feb 01, 2016
Contact name NOZOMU TAKATA
Organization name Northwestern University Feinberg School of Medicine
Department Center for Vascular and Developmental Biology
Street address 303 East Superior Street, 10-220
City Chicago
State/province Illinois
ZIP/Postal code 60611
Country USA
Platform ID GPL1261
Series (1)
GSE74236 Establishment of Functional Genomics Pipeline in Mouse Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9

Data table header descriptions

Data table
1415670_at 9.909171
1415671_at 11.50281
1415672_at 12.03464
1415673_at 10.18302
1415674_a_at 10.23441
1415675_at 9.340068
1415676_a_at 12.58162
1415677_at 8.079077
1415678_at 10.9514
1415679_at 10.94648
1415680_at 10.42992
1415681_at 10.67552
1415682_at 9.570814
1415683_at 12.22722
1415684_at 9.822107
1415685_at 10.17316
1415686_at 9.517221
1415687_a_at 11.00125
1415688_at 11.19738
1415689_s_at 8.077803

Total number of rows: 45101

Table truncated, full table size 894 Kbytes.

Supplementary file Size Download File type/resource
GSM1915105_12012001_MARGN_05_MG430_2.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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