|
Status |
Public on Feb 01, 2016 |
Title |
day0_rep1 |
Sample type |
RNA |
|
|
Source name |
mouse ES cells with SFEBq
|
Organism |
Mus musculus |
Characteristics |
cell type: differentiated mouse embryonic stem cells
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using QIAcubu’s RNA micro extraction mode (Qiagen), and cDNA was synthesized using SuperScript II Reverse Transcriptase (invitrogen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
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|
|
Hybridization protocol |
RNA was subsequently fragmented and hybridized to the GeneChip according to manufacturer’s instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
Scan protocol |
The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix).
|
Description |
Day0 sample (ES cells) from differentiated mouse ES cells with SFEBq, duplicate1
|
Data processing |
The raw data were summarized with the RMA algorithm (R 2.13.0, Bioconductor 2.8).
|
|
|
Submission date |
Oct 21, 2015 |
Last update date |
Feb 01, 2016 |
Contact name |
NOZOMU TAKATA |
E-mail(s) |
nozomu.takata@northwestern.edu
|
Organization name |
Northwestern University Feinberg School of Medicine
|
Department |
Center for Vascular and Developmental Biology
|
Street address |
303 East Superior Street, 10-220
|
City |
Chicago |
State/province |
Illinois |
ZIP/Postal code |
60611 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE74236 |
Establishment of Functional Genomics Pipeline in Mouse Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9 |
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