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Sample GSM1915108 Query DataSets for GSM1915108
Status Public on Feb 01, 2016
Title day3_rep2
Sample type RNA
 
Source name mouse ES cells with SFEBq
Organism Mus musculus
Characteristics cell type: differentiated mouse embryonic stem cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using QIAcubu’s RNA micro extraction mode (Qiagen), and cDNA was synthesized using SuperScript II Reverse Transcriptase (invitrogen).
Label biotin
Label protocol Biotinylated cRNAs were prepared according to the Affymetrix standard labeling protocol.
 
Hybridization protocol RNA was subsequently fragmented and hybridized to the GeneChip according to manufacturer’s instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix).
Description Day3 sample (epiblast-like tissue) from differentiated mouse ES cells with SFEBq, duplicate2
Data processing The raw data were summarized with the RMA algorithm (R 2.13.0, Bioconductor 2.8).
 
Submission date Oct 21, 2015
Last update date Feb 01, 2016
Contact name NOZOMU TAKATA
E-mail(s) nozomu.takata@northwestern.edu
Organization name Northwestern University Feinberg School of Medicine
Department Center for Vascular and Developmental Biology
Street address 303 East Superior Street, 10-220
City Chicago
State/province Illinois
ZIP/Postal code 60611
Country USA
 
Platform ID GPL1261
Series (1)
GSE74236 Establishment of Functional Genomics Pipeline in Mouse Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9

Data table header descriptions
ID_REF
VALUE log2 RMA

Data table
ID_REF VALUE
1415670_at 9.649861
1415671_at 11.9728
1415672_at 11.98624
1415673_at 10.22336
1415674_a_at 10.33537
1415675_at 9.49083
1415676_a_at 12.59617
1415677_at 7.821287
1415678_at 10.90353
1415679_at 11.37066
1415680_at 10.62659
1415681_at 10.75031
1415682_at 9.69161
1415683_at 12.02965
1415684_at 9.615854
1415685_at 10.02217
1415686_at 9.516434
1415687_a_at 11.0367
1415688_at 11.21021
1415689_s_at 9.118254

Total number of rows: 45101

Table truncated, full table size 894 Kbytes.




Supplementary file Size Download File type/resource
GSM1915108_12012004_MARGN_08_MG430_2.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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