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Status |
Public on Feb 01, 2016 |
Title |
BT474_1day_AICAR_rep2 |
Sample type |
RNA |
|
|
Source name |
BT474 cells treated for 24h with AICAR
|
Organism |
Homo sapiens |
Characteristics |
cell line: breast cancer cell line BT474 agent: AICAR
|
Treatment protocol |
Reagents were simply added to the media for cell treatment in regular 6cm culture dish.
|
Growth protocol |
Cells were cultured in regular DMEM. 24h after seeding, media was changed and fresh media with vehicle or AICAR 0.5mM
|
Extracted molecule |
total RNA |
Extraction protocol |
After 24h with vehicle or AICAR, RNA was purified with Aurum Total RNA Mini-Kit (Bio-Rad).
|
Label |
biotin
|
Label protocol |
Labeling was performed using standard Affymetrix protocol at Génome Québec Innovation Centre (McGill University)
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|
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Hybridization protocol |
RNA was hybridized to GeneChip® Human Genome U133 Plus 2.0 (Affymetrix) and quantified at Génome Québec Innovation Centre (McGill University)
|
Scan protocol |
Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
|
Data processing |
affymetrix-algorithm-name = rma affymetrix-algorithm-version = 1.0 program-name = Expression Console program-version = 1.3.1.187 The expression data were analyzed using FlexArray10 software.
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Submission date |
Dec 05, 2015 |
Last update date |
Feb 02, 2016 |
Contact name |
Etienne Audet-Walsh |
E-mail(s) |
etienne.audet-walsh@crchudequebec.ulaval.ca
|
Organization name |
Université Laval
|
Department |
Molecular Medicine
|
Street address |
2705 boulevard Laurier
|
City |
Québec City |
State/province |
QC |
ZIP/Postal code |
G1V 4G2 |
Country |
Canada |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE75727 |
The PGC-1α/ERRα Axis Represses One-Carbon Metabolism and Promotes Sensitivity to Anti-Folate Therapy in Breast Cancer [Microarray expression] |
GSE75877 |
The PGC-1α/ERRα Axis Represses One-Carbon Metabolism and Promotes Sensitivity to Anti-Folate Therapy in Breast Cancer |
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