|
| Status |
Public on Oct 15, 2008 |
| Title |
Carcinoma component of a progressed adenoma (P28C_s32_b21) |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
colon
|
| Organism |
Homo sapiens |
| Characteristics |
Carcinoma component of a progressed adenoma
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
as described in Weiss et al, Mol Pathol. 1999 Oct;52(5):243-51.
|
| Label |
Cy3
|
| Label protocol |
as described by Snijders et al, Nat Genetics, volume 29, p 263-4
|
| |
|
| Channel 2 |
| Source name |
female Reference DNA
|
| Organism |
Homo sapiens |
| Characteristics |
A pool of ten normal individuals
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNAzol (Invitrogen) according to manufacturer's protocol
|
| Label |
Cy5
|
| Label protocol |
as described by Snijders et al, Nat Genetics, volume 29, p 263-4
|
| |
|
| |
| Hybridization protocol |
as described by Carvalho et al, Cell Oncol. 2006;28(5-6):283-94.
|
| Scan protocol |
Microarray Scanner G2505B (Agilent Technologies), default settings
|
| Description |
n/a
|
| Data processing |
Spots were quantified in ImaGene 5.6.1 software using default settings. BAC clones were positioned along the genome according to the July 2003 freeze. After exclusion of flagged spots, the average of the triplicate spots was calculated for each BAC clone and 2log ratios (tumour/normal reference signal) were normalised by subtraction of the mode value of BAC clones on chromosome 1-22. BAC clones with flagged spots were excluded from analysis.
|
| |
|
| Submission date |
Jun 04, 2007 |
| Last update date |
Oct 15, 2008 |
| Contact name |
Daoud Sie |
| E-mail(s) |
d.sie@vumc.nl
|
| Phone |
+31 20 4442428
|
| Organization name |
Vrije Universiteit Medical Center
|
| Department |
Pathology
|
| Lab |
Microarray Core Facility
|
| Street address |
De Boelelaan 1117
|
| City |
Amsterdam |
| ZIP/Postal code |
1081 HV |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL3443 |
| Series (1) |
| GSE8067 |
Multiple genes at the chromosome 20q amplicon contribute to colorectal adenoma to carcinoma progression |
|
| Data table header descriptions |
| ID_REF |
|
| Signal Median CH1 |
spot signal channel 1 (Cy3) |
| Background Median CH1 |
background signal of channel 1 (Cy3) |
| Signal Median CH2 |
spot signal channel 2 (Cy5) |
| Background Median CH2 |
background signal of channel 1 (Cy5) |
| Log2ratio |
|
| Flag |
A flag to highlight spots that suffer from poor printing and other experimental artefacts (0 acceptable; 1-4 not acceptable) |
| VALUE |
same as UNF_VALUE but with flagged values removed |
| UNF_VALUE |
Normalized value of the log2ratio. Flagged features are excluded. Values where also deleted if the each triplicate had a Standard Deviation higher than 0.2. |
