|
| Status |
Public on Jun 21, 2007 |
| Title |
Mo17xB73 immature ear tissue, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Zea mays immature ear tissue, genotype Mo17xB73
|
| Organism |
Zea mays |
| Characteristics |
Stage: immature ear (3.7-5.4mm); Genotype: Mo17xB73
|
| Growth protocol |
Immature ears from inbreds Mo17 and B73
and hybrids Mo17xB73 and B73xMo17 were collected from
field-grown maize plants during July 2005 for gene expression
analysis. The maize plants were grown on the St. Paul campus
Agricultural Experiment Station. Ears were collected for each
genotype between 9 am and 11 am on each of three consecutive
days from various positions in the field. Ears ranging
from 3.7 to 5.4 mm in length were sampled. Ears were flash
frozen in liquid nitrogen following the manual removal of
husks and silks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For immature ear RNA isolation, tissues from 6 ears/genotype/biological replicate were pooled and ground in liquid nitrogen. RNAs were extracted using Trizol reagent according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). All RNAs were subsequently subjected to DNAse treatment and phenol:chloroform extraction. RNAs were precipitated with 0.13 volume sodium acetate (pH 5.5) and 2.53 volume ethanol. Resuspended RNAs were purified further using the RNeasy system, according to the manufacturer’s instructions (QIAGEN, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Eight ug of total RNA was labeled for each hybridization using the One-Cycle cDNA Synthesis Kit, according to the manufacturer’s instructions (Affymetrix, Santa Clara CA).
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the recommended Affymetrix protocols at the University of Minnesota Microarray facility.
|
| Scan protocol |
The Genechip 3000 scanner was used to scan each array
|
| Description |
refer to PMID: 16702414
|
| Data processing |
MAS5.0 values are reported; the .cel file is also available
|
| |
|
| Submission date |
Jun 19, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Nathan M Springer |
| E-mail(s) |
springer@umn.edu
|
| Phone |
6126246241
|
| Fax |
6126251738
|
| Organization name |
University of Minnesota
|
| Department |
Plant Biology
|
| Street address |
1445 Gortner Ave
|
| City |
Saint Paul |
| State/province |
MN |
| ZIP/Postal code |
55108 |
| Country |
USA |
| |
|
| Platform ID |
GPL4032 |
| Series (2) |
| GSE8176 |
Cis-transcriptional variation in maize inbred lines B73 and Mo17 leads to additive expression - Immature ear data |
| GSE8194 |
Cis-transcriptional variation in maize inbred lines B73 and Mo17 leads to additive expression |
|
